Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Genetic Manipulations and Plasmid Construction
2.3. Fluorescence Analysis
2.4. Real-Time Quantitative PCR (RT-qPCR)
2.5. Batch and Fed-Batch Cultivation
2.6. Analytical Methods
2.7. Data Availability
3. Result
3.1. Gene Expression and Construction of the Modified Glycolate Pathway
3.2. Replacement of PTet with the Synthetic Promoter PL3429
3.3. Optimizing the Different Synthetic Promoters for Glycolate Production
3.4. Production of Glycolate in 5-L Bioreactor
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhao, M.; Shang, J.; Chen, J.; Zabed, H.M.; Qi, X. Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters. Fermentation 2024, 10, 67. https://doi.org/10.3390/fermentation10010067
Zhao M, Shang J, Chen J, Zabed HM, Qi X. Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters. Fermentation. 2024; 10(1):67. https://doi.org/10.3390/fermentation10010067
Chicago/Turabian StyleZhao, Mei, Jie Shang, Jiaojiao Chen, Hossain M. Zabed, and Xianghui Qi. 2024. "Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters" Fermentation 10, no. 1: 67. https://doi.org/10.3390/fermentation10010067