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Article

Hsa-miR-183-5p Modulates Cell Adhesion by Repression of ITGB1 Expression in Prostate Cancer

1
Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay
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Departamento de Genética, Facultad de Medicina, Universidad de la República, Av. Gral. Flores 2125, Montevideo 11800, Uruguay
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Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay
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Departamento de Biología Celular, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo 11400, Uruguay
*
Author to whom correspondence should be addressed.
Present address: Departamento Básico de Medicina, Hospital de Clínicas/Facultad de Medicina, Universidad de la República, Av. Italia s/n, Piso 15, Montevideo 11600, Uruguay.
Academic Editor: Luca Falzone
Non-Coding RNA 2022, 8(1), 11; https://doi.org/10.3390/ncrna8010011
Received: 22 December 2021 / Revised: 5 January 2022 / Accepted: 8 January 2022 / Published: 18 January 2022
(This article belongs to the Special Issue Feature Papers from Non-coding RNA Reviewers)
Prostate cancer is a major health problem worldwide. MiR-183 is an oncomiR and a candidate biomarker in prostate cancer, affecting various pathways responsible for disease initiation and progression. We sought to discover the most relevant processes controlled by miR-183 through an unbiased transcriptomic approach using prostate cell lines and patient tissues to identify miR-183 responsive genes and pathways. Gain of function experiments, reporter gene assays, and transcript and protein measurements were conducted to validate predicted functional effects and protein mediators. A total of 135 candidate miR-183 target genes overrepresenting cell adhesion terms were inferred from the integrated transcriptomic analysis. Cell attachment, spreading assays and focal adhesion quantification of miR-183-overexpressing cells confirmed the predicted reduction in cell adhesion. ITGB1 was validated as a major target of repression by miR-183 as well as a mediator of cell adhesion in response to miR-183. The reporter gene assay and PAR-CLIP read mapping suggest that ITGB1 may be a direct target of miR-183. The negative correlation between miR-183 and ITGB1 expression in prostate cancer cohorts supports their interaction in the clinical set. Overall, cell adhesion was uncovered as a major pathway controlled by miR-183 in prostate cancer, and ITGB1 was identified as a relevant mediator of this effect. View Full-Text
Keywords: cancer; microRNA; focal adhesion; miR-183; prostate; ITGB1; TCGA; AGO-PAR-CLIP cancer; microRNA; focal adhesion; miR-183; prostate; ITGB1; TCGA; AGO-PAR-CLIP
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MDPI and ACS Style

Oliveira-Rizzo, C.; Ottati, M.C.; Fort, R.S.; Chavez, S.; Trinidad, J.M.; DiPaolo, A.; Garat, B.; Sotelo-Silveira, J.R.; Duhagon, M.A. Hsa-miR-183-5p Modulates Cell Adhesion by Repression of ITGB1 Expression in Prostate Cancer. Non-Coding RNA 2022, 8, 11. https://doi.org/10.3390/ncrna8010011

AMA Style

Oliveira-Rizzo C, Ottati MC, Fort RS, Chavez S, Trinidad JM, DiPaolo A, Garat B, Sotelo-Silveira JR, Duhagon MA. Hsa-miR-183-5p Modulates Cell Adhesion by Repression of ITGB1 Expression in Prostate Cancer. Non-Coding RNA. 2022; 8(1):11. https://doi.org/10.3390/ncrna8010011

Chicago/Turabian Style

Oliveira-Rizzo, Carolina, María C. Ottati, Rafael S. Fort, Santiago Chavez, Juan M. Trinidad, Andrés DiPaolo, Beatriz Garat, José R. Sotelo-Silveira, and María A. Duhagon. 2022. "Hsa-miR-183-5p Modulates Cell Adhesion by Repression of ITGB1 Expression in Prostate Cancer" Non-Coding RNA 8, no. 1: 11. https://doi.org/10.3390/ncrna8010011

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