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Non-Coding RNA, Volume 11, Issue 6 (December 2025) – 1 article

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10 pages, 991 KB  
Perspective
Exploring microRNAs, One Cell at a Time
by Jessica Kreutz, Tijana Mitić and Andrea Caporali
Non-Coding RNA 2025, 11(6), 73; https://doi.org/10.3390/ncrna11060073 (registering DOI) - 22 Oct 2025
Abstract
The emergence of single-cell sequencing and computational analysis has dramatically improved our understanding of cellular diversity and gene expression dynamics. The rapid advancement of high-throughput omics technologies has led to an exponential growth in biological data. However, many gene regulatory processes at the [...] Read more.
The emergence of single-cell sequencing and computational analysis has dramatically improved our understanding of cellular diversity and gene expression dynamics. The rapid advancement of high-throughput omics technologies has led to an exponential growth in biological data. However, many gene regulatory processes at the single-cell level remain underexplored, especially those regulated by post-transcriptional mechanisms involving microRNAs (miRNAs). miRNAs are essential regulators of gene expression, affecting cellular functions in both normal and disease states. Recent innovations, such as single-cell gene expression profiling and bioinformatic analysis, have enabled comprehensive studies that uncover previously hidden miRNA profiles. In this context, we present experimental tools and computational methods for analysing cell-specific miRNA abundance and investigating their mechanisms. These approaches are expected to reveal the complex nature of miRNA biology and, more broadly, enhance our understanding of life sciences and diseases. Full article
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