Gelatin Nanoparticles for Targeted Dual Drug Release out of Alginate-di-Aldehyde-Gelatin Gels
Abstract
:1. Introduction
2. Results
2.1. Manufacturing Process of GNPs
2.2. Inclusion Capacity
2.3. Biocompatibility
2.3.1. Live Dead Staining
2.3.2. Cell Proliferation Assay (WST-I)
2.3.3. Lactate Dehydrogenase (LDH)
2.4. Drug Release Experiments
2.4.1. Clindamycin Release out of ADA-GEL Beads
2.4.2. FITC-Protein a Release out of GNPs within ADA-GEL Beads
2.4.3. BMP-2 Release out of GNPs within ADA-GEL Beads
3. Discussion
3.1. Characterization of GNP
3.2. Inclusion Capacity
3.3. Biocompatibility
3.4. Drug Release Experiments
4. Conclusions
5. Materials and Methods
5.1. Reagents and Materials
5.2. Manufacturing of the Gelatin Nanoparticles (GNPs)
Manufacturing of FITC-Protein A-Loaded GNPs and BMP-2 Loaded GNPs
5.3. Characterization of the GNP
5.3.1. Characterization of GNP by ESEM
5.3.2. Determination of the Inclusion Capacity
5.4. Biocompatibility
5.4.1. Live/Dead Assay
5.4.2. Cell Proliferation Assay
5.4.3. Lactate Dehydrogenase (LDH) Assay
5.5. ADA-GEL Hydrogel
5.5.1. Preparation of Alginate-di-Aldehyde (ADA)
5.5.2. Crosslinking ADA and GEL
5.6. ADA-GEL Beads with Drugs
5.6.1. ADA-GEL Beads with Clindamycin and FITC-Protein A-Containing GNPs
5.6.2. ADA-GEL Beads with CLI and BMP-2 Containing GNP
5.7. Drug Release Experiments
5.7.1. Drug Release from ADA-GEL Beads
5.7.2. Dual Drug Release from ADA-GEL Beads
5.7.3. Quantitative Analysis
5.7.4. Kinetics Model
5.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schrade, S.; Ritschl, L.; Süss, R.; Schilling, P.; Seidenstuecker, M. Gelatin Nanoparticles for Targeted Dual Drug Release out of Alginate-di-Aldehyde-Gelatin Gels. Gels 2022, 8, 365. https://doi.org/10.3390/gels8060365
Schrade S, Ritschl L, Süss R, Schilling P, Seidenstuecker M. Gelatin Nanoparticles for Targeted Dual Drug Release out of Alginate-di-Aldehyde-Gelatin Gels. Gels. 2022; 8(6):365. https://doi.org/10.3390/gels8060365
Chicago/Turabian StyleSchrade, Sophie, Lucas Ritschl, Regine Süss, Pia Schilling, and Michael Seidenstuecker. 2022. "Gelatin Nanoparticles for Targeted Dual Drug Release out of Alginate-di-Aldehyde-Gelatin Gels" Gels 8, no. 6: 365. https://doi.org/10.3390/gels8060365