Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Alginate Hydrogels
2.2. Cytotoxicity Assessment of Alginate Hydrogels
2.3. Alginate Hydrogels Specific Binding of Proteins
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Alginate Hydrogel Synthesis
4.3. Cell Culture and Cytotoxicity Assay
4.4. Specific His-Tagged Protein Binding Assessment
4.5. Protein Binding Capacity Assay
4.6. Optical and Fluorescence Imaging Investigation of Dried or Lyophilized Hydrogels
4.7. AFM Surface Characterization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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EGFP-HisTAG (µg) | Incubation Time (h) | |
---|---|---|
t1 = 1 h | t2 = 24 h | |
12.5 µg | 98.45% | 95.05% |
25 µg | 97.15% | 87.01% |
37.5 µg | 93.52% | 67.16% |
50 µg | 87.28% | 56.11% |
62.5 µg | 73.56% | 43.14% |
EGFP-HisTAG (µg) | Incubation Time (h) | |
---|---|---|
t1 = 1 h | t2 = 24 h | |
12.5 µg | 96.87% | 78.50% |
25 µg | 85.91% | 67.50% |
37.5 µg | 77.24% | 59.77% |
50 µg | 71.92% | 57.44% |
62.5 µg | 66.40% | 52.35% |
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Dumitrașcu, A.-M.; Caraș, I.; Țucureanu, C.; Ermeneanu, A.-L.; Tofan, V.-C. Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins. Gels 2022, 8, 66. https://doi.org/10.3390/gels8020066
Dumitrașcu A-M, Caraș I, Țucureanu C, Ermeneanu A-L, Tofan V-C. Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins. Gels. 2022; 8(2):66. https://doi.org/10.3390/gels8020066
Chicago/Turabian StyleDumitrașcu, Andrei-Mihai, Iuliana Caraș, Cătălin Țucureanu, Andreea-Laura Ermeneanu, and Vlad-Constantin Tofan. 2022. "Nickel (II) and Cobalt (II) Alginate Biopolymers as a “Carry and Release” Platform for Polyhistidine-Tagged Proteins" Gels 8, no. 2: 66. https://doi.org/10.3390/gels8020066