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Article

The Colletotrichum siamense Hydrophobin CsHydr1 Interacts with the Lipid Droplet-Coating Protein CsCap20 and Regulates Lipid Metabolism and Virulence

by
Na Wang
†,
Jiyuan Wang
†,
Jingwen Lu
,
Yu Liu
,
Yitao Xi
,
Miao Song
,
Xiaoling Guan
,
Zhigang Li
,
Xiao Li
,
Yu Zhang
,
Chunhua Lin
* and
Weiguo Miao
*
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
J. Fungi 2022, 8(9), 977; https://doi.org/10.3390/jof8090977
Submission received: 31 July 2022 / Revised: 5 September 2022 / Accepted: 15 September 2022 / Published: 19 September 2022
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Abstract

Previous studies of the lipid droplet-coating protein Cap20 in Colletotrichum show that it plays a key role in appressorium development and virulence. In this study, the hydrophobin CsHydr1, which contains a signal peptide of 19 amino acids and a hydrophobic domain (HYDRO), was shown to interact with CsCap20 in Colletotrichum siamense. The CsHydr1 deletion mutant showed slightly enhanced mycelial growth, small conidia, slow spore germination and appressoria formation, cell wall integrity and virulence. Like CsCAP20, CsHydr1 is also localized on the lipid droplet surface of C. siamense. However, when CsCap20 was absent, some CsHydr1 was observed in other parts. Quantitative lipid determination showed that the absence of either CsHydr1 or CsCap20 reduced the content of lipids in mycelia and conidia, while the effect of CsCap20 was more obvious; these results suggest that an interaction protein CsHydr1 of CsCap20 is localized on the lipid droplet surface and involved in lipid metabolism, which affects appressorium formation and virulence in C. siamense.
Keywords: Colletotrichum siamense; hydrophobin CsHydr1; lipid droplet-coating protein CsCap20; appressoriual turgor pressure; virulence Colletotrichum siamense; hydrophobin CsHydr1; lipid droplet-coating protein CsCap20; appressoriual turgor pressure; virulence

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MDPI and ACS Style

Wang, N.; Wang, J.; Lu, J.; Liu, Y.; Xi, Y.; Song, M.; Guan, X.; Li, Z.; Li, X.; Zhang, Y.; et al. The Colletotrichum siamense Hydrophobin CsHydr1 Interacts with the Lipid Droplet-Coating Protein CsCap20 and Regulates Lipid Metabolism and Virulence. J. Fungi 2022, 8, 977. https://doi.org/10.3390/jof8090977

AMA Style

Wang N, Wang J, Lu J, Liu Y, Xi Y, Song M, Guan X, Li Z, Li X, Zhang Y, et al. The Colletotrichum siamense Hydrophobin CsHydr1 Interacts with the Lipid Droplet-Coating Protein CsCap20 and Regulates Lipid Metabolism and Virulence. Journal of Fungi. 2022; 8(9):977. https://doi.org/10.3390/jof8090977

Chicago/Turabian Style

Wang, Na, Jiyuan Wang, Jingwen Lu, Yu Liu, Yitao Xi, Miao Song, Xiaoling Guan, Zhigang Li, Xiao Li, Yu Zhang, and et al. 2022. "The Colletotrichum siamense Hydrophobin CsHydr1 Interacts with the Lipid Droplet-Coating Protein CsCap20 and Regulates Lipid Metabolism and Virulence" Journal of Fungi 8, no. 9: 977. https://doi.org/10.3390/jof8090977

APA Style

Wang, N., Wang, J., Lu, J., Liu, Y., Xi, Y., Song, M., Guan, X., Li, Z., Li, X., Zhang, Y., Lin, C., & Miao, W. (2022). The Colletotrichum siamense Hydrophobin CsHydr1 Interacts with the Lipid Droplet-Coating Protein CsCap20 and Regulates Lipid Metabolism and Virulence. Journal of Fungi, 8(9), 977. https://doi.org/10.3390/jof8090977

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