Involvement of the Autophagy Protein Atg1 in Development and Virulence in Botryosphaeria dothidea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Culture Conditions
2.2. Gene Deletion and Complementation
2.3. Staining and Microscopy
2.4. Protein Manipulation and Western Blotting
2.5. Pathogenicity Tests
2.6. Transcriptome Sequencing and Analysis
3. Results
3.1. Identification of BdATG1 in B. dothidea
3.2. Deletion and Complementation of BdATG1 in B. dothidea
3.3. BdAtg1 Is Indispensable for Autophagy
3.4. BdAtg1 Is Involved in Vegetative Growth
3.5. BdAtg1 Is Crucial for Asexual and Sexual Reproduction
3.6. BdAtg1 Is Required for Full Virulence
3.7. BdAtg1 Mediates Nuclei Distribution
3.8. BdAtg1 Influences Multiple Metabolic Pathways
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, N.; Zhu, M.; Zhang, Y.; Wang, Z.; Li, B.; Ren, W. Involvement of the Autophagy Protein Atg1 in Development and Virulence in Botryosphaeria dothidea. J. Fungi 2022, 8, 904. https://doi.org/10.3390/jof8090904
Liu N, Zhu M, Zhang Y, Wang Z, Li B, Ren W. Involvement of the Autophagy Protein Atg1 in Development and Virulence in Botryosphaeria dothidea. Journal of Fungi. 2022; 8(9):904. https://doi.org/10.3390/jof8090904
Chicago/Turabian StyleLiu, Na, Meiqi Zhu, Yihan Zhang, Zhongqiang Wang, Baohua Li, and Weichao Ren. 2022. "Involvement of the Autophagy Protein Atg1 in Development and Virulence in Botryosphaeria dothidea" Journal of Fungi 8, no. 9: 904. https://doi.org/10.3390/jof8090904