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Article

Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184

by
Fabiola Altimira
1,*,
Matias Arias-Aravena
1,
Ling Jian
2,
Nicolas Real
1,
Pablo Correa
1,
Carolina González
3,
Sebastián Godoy
1,
Jean Franco Castro
4,
Olga Zamora
5,
Cristina Vergara
5,
Nancy Vitta
1 and
Eduardo Tapia
1
1
Laboratorio de Entomología y Biotecnología, Instituto de Investigaciones Agropecuarias, INIA La Platina, Santiago 8831314, Chile
2
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Center for Bioinformatics and Genome Biology, Fundación Ciencia & Vida, Santiago 7780272, Chile
4
Banco de Recursos Genéticos Microbianos, Instituto de Investigaciones Agropecuarias, INIA, Chillán 3800062, Chile
5
Laboratorio de Materias Primas y Alimentos, Instituto de Investigaciones Agropecuarias, INIA La Platina, Santiago 8831314, Chile
*
Author to whom correspondence should be addressed.
J. Fungi 2022, 8(3), 253; https://doi.org/10.3390/jof8030253
Submission received: 7 February 2022 / Revised: 22 February 2022 / Accepted: 26 February 2022 / Published: 1 March 2022
(This article belongs to the Section Fungi in Agriculture and Biotechnology)
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Abstract

The entomopathogenic fungus Beauveria pseudobassiana strain RGM 2184 can reach a maximum efficacy of 80% against the quarantine pest Lobesia botrana in field assays. In this study, the RGM 2184 genome was sequenced, and genome mining analyses were performed to predict the factors involved in its insecticidal activity. Additionally, the metabolic profiling of the RMG 2184 culture’s supernatants was analyzed by mass spectrometry, and the insecticidal activity from one of these extracts was evaluated in Galleria mellonella larvae. The genome analysis resulted in 114 genes encoding for extracellular enzymes, four biosynthetic gene clusters reported as producers of insecticidal and bactericidal factors (oosporein, beauvericin, desmethylbassianin, and beauveriolide), 20 toxins, and at least 40 undescribed potential biocontrol factors (polyketides and nonribosomal peptides). Comparative genomic analysis revealed that 65–95% of these genes are Beauveria genus-specific. Metabolic profiling of supernatant extracts from RGM 2184 cultures exhibited secondary metabolites such as beauveriolide, oosporein, inflatin C, and bassiatin. However, a number of detected metabolites still remain undescribed. The metabolite extract caused 79% mortality of Galleria mellonella larvae at 28 days. The results of this research lay the groundwork for the study of new insecticidal molecules.
Keywords: Beauveria pseudobassiana; entomopathogenic fungi (EPF); nonribosomal peptide synthetase (NRPS); polyketide synthase (PKS); extracellular enzymes; insecticidal activity Beauveria pseudobassiana; entomopathogenic fungi (EPF); nonribosomal peptide synthetase (NRPS); polyketide synthase (PKS); extracellular enzymes; insecticidal activity
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MDPI and ACS Style

Altimira, F.; Arias-Aravena, M.; Jian, L.; Real, N.; Correa, P.; González, C.; Godoy, S.; Castro, J.F.; Zamora, O.; Vergara, C.; et al. Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184. J. Fungi 2022, 8, 253. https://doi.org/10.3390/jof8030253

AMA Style

Altimira F, Arias-Aravena M, Jian L, Real N, Correa P, González C, Godoy S, Castro JF, Zamora O, Vergara C, et al. Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184. Journal of Fungi. 2022; 8(3):253. https://doi.org/10.3390/jof8030253

Chicago/Turabian Style

Altimira, Fabiola, Matias Arias-Aravena, Ling Jian, Nicolas Real, Pablo Correa, Carolina González, Sebastián Godoy, Jean Franco Castro, Olga Zamora, Cristina Vergara, and et al. 2022. "Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184" Journal of Fungi 8, no. 3: 253. https://doi.org/10.3390/jof8030253

APA Style

Altimira, F., Arias-Aravena, M., Jian, L., Real, N., Correa, P., González, C., Godoy, S., Castro, J. F., Zamora, O., Vergara, C., Vitta, N., & Tapia, E. (2022). Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic Fungus Beauveria pseudobassiana RGM 2184. Journal of Fungi, 8(3), 253. https://doi.org/10.3390/jof8030253

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