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Article

Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626

1
Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
2
Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
3
Associate Laboratory i4HB—Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal
4
US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
5
Department of Agricultural Biology, Colorado State University, Fort Collins, CO 80523, USA
6
Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Ricardo Franco-Duarte and Celia Pais
J. Fungi 2022, 8(3), 254; https://doi.org/10.3390/jof8030254
Received: 30 January 2022 / Revised: 26 February 2022 / Accepted: 27 February 2022 / Published: 1 March 2022
(This article belongs to the Special Issue New Trends in Yeast Genomics)
The ascomycetous yeast Candida membranifaciens has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated four C. membranifaciens strains from soil and investigated their potential to overproduce riboflavin. C. membranifaciens IST 626 was found to produce the highest concentrations of riboflavin. The volumetric production of this vitamin was higher when C. membranifaciens IST 626 cells were cultured in a commercial medium without iron and when xylose was the available carbon source compared to the same basal medium with glucose. Supplementation of the growth medium with 2 g/L glycine favored the metabolization of xylose, leading to biomass increase and consequent enhancement of riboflavin volumetric production that reached 120 mg/L after 216 h of cultivation. To gain new insights into the molecular basis of riboflavin production and carbon source utilization in this species, the first annotated genome sequence of C. membranifaciens is reported in this article, as well as the result of a comparative genomic analysis with other relevant yeast species. A total of 5619 genes were predicted to be present in C. membranifaciens IST 626 genome sequence (11.5 Mbp). Among them are genes involved in riboflavin biosynthesis, iron homeostasis, and sugar uptake and metabolism. This work put forward C. membranifaciens IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species. View Full-Text
Keywords: Candida membranifaciens; Debaryomycetaceae; yeast isolation; soil; riboflavin; flavinogenic yeasts; iron metabolism; xylose assimilation Candida membranifaciens; Debaryomycetaceae; yeast isolation; soil; riboflavin; flavinogenic yeasts; iron metabolism; xylose assimilation
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MDPI and ACS Style

Palma, M.; Mondo, S.; Pereira, M.; Vieira, É.; Grigoriev, I.V.; Sá-Correia, I. Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626. J. Fungi 2022, 8, 254. https://doi.org/10.3390/jof8030254

AMA Style

Palma M, Mondo S, Pereira M, Vieira É, Grigoriev IV, Sá-Correia I. Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626. Journal of Fungi. 2022; 8(3):254. https://doi.org/10.3390/jof8030254

Chicago/Turabian Style

Palma, Margarida, Stephen Mondo, Mariana Pereira, Érica Vieira, Igor V. Grigoriev, and Isabel Sá-Correia. 2022. "Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626" Journal of Fungi 8, no. 3: 254. https://doi.org/10.3390/jof8030254

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