Next Article in Journal
Pathogenesis, Immunology and Management of Dermatophytosis
Previous Article in Journal
Molecular Evolution of Lysine Biosynthesis in Agaricomycetes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
JoF
Volume 8
Issue 1
10.3390/jof8010038
jof-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism in Pestalotiopsis microspora

by
Mengkai Zhou
1,
Ze Li
1,
Yanjie Liu
1,
Ping Zhang
1,
Xiaoran Hao
2 and
Xudong Zhu
1,*
1
Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing 100875, China
2
National Experimental Teaching Demonstrating Center, College of Life Sciences, Beijing Normal University, Beijing 100875, China
*
Author to whom correspondence should be addressed.
J. Fungi 2022, 8(1), 38; https://doi.org/10.3390/jof8010038
Submission received: 8 December 2021 / Revised: 28 December 2021 / Accepted: 29 December 2021 / Published: 31 December 2021
Browse Figures
Versions Notes

Abstract

Melanins are the common fungal pigment, which contribute to stress resistance and pathogenesis. However, few studies have explored the regulation mechanism of its synthesis in filamentous fungi. In this study, we identified two transcription factors, Pmr1 and Pmr2, in the filamentous fungus Pestalotiopsis microspora. Computational and phylogenetic analyses revealed that Pmr1 and Pmr2 were located in the gene cluster for melanin biosynthesis. The targeted deletion mutant strain Δpmr1 displayed defects in biosynthesis of conidia pigment and morphological integrity. The deletion of pmr2 resulted in reduced conidia pigment, but the mycelial morphology had little change. Moreover, Δpmr2 produced decreased conidia. RT-qPCR data revealed that expression levels of genes in the melanin biosynthesis gene cluster were downregulated from the loss of Pmr1 and Pmr2. Interestingly, the yield of secondary metabolites in the mutant strains Δpmr1 and Δpmr2 increased, comparing with the wild type, and additionally, Pmr1 played a larger regulatory role in secondary metabolism. Taken together, our results revealed the crucial roles of the transcription factors Pmr1 and Pmr2 in melanin synthesis, asexual development and secondary metabolism in the filamentous fungus P. microspora.
Keywords: transcription factor; melanin; conidia development; Pestalotiopsis microspora transcription factor; melanin; conidia development; Pestalotiopsis microspora

Share and Cite

MDPI and ACS Style

Zhou, M.; Li, Z.; Liu, Y.; Zhang, P.; Hao, X.; Zhu, X. Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism in Pestalotiopsis microspora. J. Fungi 2022, 8, 38. https://doi.org/10.3390/jof8010038

AMA Style

Zhou M, Li Z, Liu Y, Zhang P, Hao X, Zhu X. Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism in Pestalotiopsis microspora. Journal of Fungi. 2022; 8(1):38. https://doi.org/10.3390/jof8010038

Chicago/Turabian Style

Zhou, Mengkai, Ze Li, Yanjie Liu, Ping Zhang, Xiaoran Hao, and Xudong Zhu. 2022. "Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism in Pestalotiopsis microspora" Journal of Fungi 8, no. 1: 38. https://doi.org/10.3390/jof8010038

APA Style

Zhou, M., Li, Z., Liu, Y., Zhang, P., Hao, X., & Zhu, X. (2022). Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism in Pestalotiopsis microspora. Journal of Fungi, 8(1), 38. https://doi.org/10.3390/jof8010038

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop