Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Candida Albicans Strain and Culture Conditions
2.2. Dendritic Compounds
2.3. Antifungal and Antibiofilm Formation Susceptibility Test
2.4. In Vitro Antibiofilm Susceptibility Test against Stablished Biofilms
2.5. Combination Therapy of Dendron ArCO2G2(SNMe3I)4 (2) with AgNO3 and EDTA against C. albicans
2.6. Resazurin Assay
2.7. Drop Plate Method
2.8. Cytotoxicity Evaluation
2.9. Ultrastructural Study
2.10. Statistical Analysis
3. Results and Discussion
3.1. Effective Prevention of C. albicans Biofilm Formation
3.2. Antibiofilm Activity against Established Biofilms
3.3. Combined Activity of ArCO2G2(SNMe3I)4 (2) and AgNO3
3.4. Combined Activity of ArCO2G2(SNMe3I)4 (2) and EDTA
3.5. Cytotoxicity
3.6. Alterations Produced in C. albicans Cells on Biofilms Due to ArCO2G2(SNMe3I)4 (2) Activity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Dendrons | Biofilm Formation | Stablished Biofilm | ||
---|---|---|---|---|
MBIC (mg/L) | MFC * (mg/L) | MBDC (mg/L) | MBEC * (mg/L) | |
ArCO2G1(SNMe3I)2 (1) | 256 | 256 | >1024 | BNE |
ArCO2G2(SNMe3I)4 (2) | 16 | 16 | 64 | BNE |
ArCO2G3(SNMe3I)8 (3) | 256 | 256 | 256 | BNE |
AgNO3 | 8 | 8–16 | 32 | BNE |
EDTA | >512 | >512 | >1024 | BNE |
Biofilm formation | ArCO2G2(SNMe3I)4(2) and AgNO3 | ArCO2G2(SNMe3I)4(2) and EDTA | ||||
(2):AgNO3 (mg/L) | Viability % | MFC * | (2):EDTA (mg/L) | Viability% | MFC * | |
8:4 | 11.61 ± 2.58% | BNE | 8:256 (MFC) | 0.00 ± 0.00% | BE | |
8:0.5 | 51.13 ± 12.7% | BNE | 8:32 | 24.08 ± 7.32% | BNE | |
Established biofilm | ArCO2G2(SNMe3I)4(2) and AgNO3 | ArCO2G2(SNMe3I)4(2) and EDTA | ||||
(2):AgNO3 (mg/L) | Viability % | MBEC * | (2):EDTA (mg/L) | Viability% | MBEC * | |
32:32 (MBEC) | 0 ± 0.00% | BE | 256:16 (MBEC) | 0 ± 0.00% | BE | |
32:16 | 42.59 ± 3.4% | BNE |
Combination | Viability% |
---|---|
ArCO2G2(SNMe3I)4 (2):AgNO3 (32:32) | 1.32 ± 0.63 |
ArCO2G2(SNMe3I)4 (2):AgNO3 (8:4) | 1.72 ± 0.68 |
ArCO2G2(SNMe3I)4 (2):AgNO3 (8:0.5) | 51.91 ± 3.73 |
ArCO2G2(SNMe3I)4 (2):EDTA (256:16) | 1.73 ± 0.10 |
ArCO2G2(SNMe3I)4 (2):EDTA (32:16) | 7.85 ± 0.21 |
ArCO2G2(SNMe3I)4 (2):EDTA (8:256) | 56.46 ± 4.35 |
ArCO2G2(SNMe3I)4 (2):EDTA (8:32) | 61.91 ± 3.24 |
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Gómez-Casanova, N.; Lozano-Cruz, T.; Soliveri, J.; Gomez, R.; Ortega, P.; Copa-Patiño, J.L.; Heredero-Bermejo, I. Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA. J. Fungi 2021, 7, 574. https://doi.org/10.3390/jof7070574
Gómez-Casanova N, Lozano-Cruz T, Soliveri J, Gomez R, Ortega P, Copa-Patiño JL, Heredero-Bermejo I. Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA. Journal of Fungi. 2021; 7(7):574. https://doi.org/10.3390/jof7070574
Chicago/Turabian StyleGómez-Casanova, Natalia, Tania Lozano-Cruz, Juan Soliveri, Rafael Gomez, Paula Ortega, José Luis Copa-Patiño, and Irene Heredero-Bermejo. 2021. "Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA" Journal of Fungi 7, no. 7: 574. https://doi.org/10.3390/jof7070574