Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolates
2.2. Fungal Strain Cultivation
2.3. Extraction of Siderophores and Calibration
2.4. Liquid Chromatography and Mass Spectrometry
2.5. Statistical Analysis
3. Results
3.1. Pigment Secretion Is Observed by Virus-Infected but Not VF A. fumigatus
3.2. Monitoring Extracellular Secretion Kinetics Reveals Differential Secretion of Siderophores
3.3. Fungal Pellets but Not Conidia Have Different HFC/FC Ratios
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Designation | Conidia $ (×108) | Cdw (mg) # |
---|---|---|---|
18–42 (VF) | UK Af293 cured from AfuPmV-1 | 7.03 | 55.3 ± 3.4 |
18–95 | UK Af293 with AfuPmV-1 | 2.75 | 42.6 ± 3.9 |
10–53 | USA Af293 with AfuPmV-1 | 2.30 | 42.1 ± 0.5 |
19–40 | 18–42 re-infected with AfuPmV-1 | 2.25 | 43.1 ± 3.3 |
19–42 | 18–42 re-infected with AfuPmV-1 | 1.84 | 42.5 ± 3.9 |
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Patil, R.H.; Kotta-Loizou, I.; Palyzová, A.; Pluháček, T.; Coutts, R.H.A.; Stevens, D.A.; Havlíček, V. Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics. J. Fungi 2021, 7, 497. https://doi.org/10.3390/jof7070497
Patil RH, Kotta-Loizou I, Palyzová A, Pluháček T, Coutts RHA, Stevens DA, Havlíček V. Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics. Journal of Fungi. 2021; 7(7):497. https://doi.org/10.3390/jof7070497
Chicago/Turabian StylePatil, Rutuja H., Ioly Kotta-Loizou, Andrea Palyzová, Tomáš Pluháček, Robert H. A. Coutts, David A. Stevens, and Vladimír Havlíček. 2021. "Freeing Aspergillus fumigatus of Polymycovirus Infection Renders It More Resistant to Competition with Pseudomonas aeruginosa Due to Altered Iron-Acquiring Tactics" Journal of Fungi 7, no. 7: 497. https://doi.org/10.3390/jof7070497