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Article

Heterologous Expression, Engineering and Characterization of a Novel Laccase of Agrocybe pediades with Promising Properties as Biocatalyst

Centro de Investigaciones Biológicas Margarita Salas, CSIC. Ramiro de Maeztu 9, 28040 Madrid, Spain
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Academic Editor: Craig Faulds
J. Fungi 2021, 7(5), 359; https://doi.org/10.3390/jof7050359
Received: 29 March 2021 / Revised: 23 April 2021 / Accepted: 29 April 2021 / Published: 4 May 2021
(This article belongs to the Special Issue Exploiting Fungal Solutions for Today's Challenges)
Agaricomycetes fungi responsible for decay of wood and other lignocellulosic substrates constitute a valuable source of lignin-degrading enzymes. Among these enzymes, laccases (multi-copper oxidases) present remarkable biotechnological potential as environmentally friendly biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the only requirement. Laccases from saprotrophic Agaricales species have been much less studied than laccases from Polyporales, despite the fact that the former fungi are excellent sources of laccases. Here, the gene of a novel laccase of Agrocybe pediades, that is secreted by the fungus during lignocellulose degradation, was synthesised de novo and expressed in Saccharomyces cerevisiae using an improved signal peptide previously obtained and enzyme directed evolution. The characterization of the new laccase variants provided new insights on the contribution of different amino acid residues to modulate laccase production, catalytic activity or optimal pH. The selected double-mutated variant also showed interesting properties as a biocatalyst, such as the ability to oxidise a wide range of substrates, including high-redox potential mediators and recalcitrant organic dyes, improved activity at neutral pH and high tolerance to inhibitors. Finally, we demonstrate the existence of three N-glycosylation sites in the laccase and their distinct effect on the secretion or catalytic activity of the enzyme. View Full-Text
Keywords: laccase; Agaricales; heterologous expression; S. cerevisiae; enzyme directed evolution; N-glycosylation; biocatalysis laccase; Agaricales; heterologous expression; S. cerevisiae; enzyme directed evolution; N-glycosylation; biocatalysis
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MDPI and ACS Style

Aza, P.; Molpeceres, G.; Ruiz-Dueñas, F.J.; Camarero, S. Heterologous Expression, Engineering and Characterization of a Novel Laccase of Agrocybe pediades with Promising Properties as Biocatalyst. J. Fungi 2021, 7, 359. https://doi.org/10.3390/jof7050359

AMA Style

Aza P, Molpeceres G, Ruiz-Dueñas FJ, Camarero S. Heterologous Expression, Engineering and Characterization of a Novel Laccase of Agrocybe pediades with Promising Properties as Biocatalyst. Journal of Fungi. 2021; 7(5):359. https://doi.org/10.3390/jof7050359

Chicago/Turabian Style

Aza, Pablo, Gonzalo Molpeceres, Francisco J. Ruiz-Dueñas, and Susana Camarero. 2021. "Heterologous Expression, Engineering and Characterization of a Novel Laccase of Agrocybe pediades with Promising Properties as Biocatalyst" Journal of Fungi 7, no. 5: 359. https://doi.org/10.3390/jof7050359

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