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Article

Colonisation and Transmission Dynamics of Candida auris among Chronic Respiratory Diseases Patients Hospitalised in a Chest Hospital, Delhi, India: A Comparative Analysis of Whole Genome Sequencing and Microsatellite Typing

1
Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India
2
Department of Zoology, Ramjas College, University of Delhi, Delhi 110007, India
3
Department of Biology, McMaster University, Hamilton, ON L8S 4K1, Canada
4
Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, 6532 SZ Nijmegen, The Netherlands
5
Centre of Expertise in Mycology Radboudumc/CWZ, 6532 SZ Nijmegen, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: David S. Perlin
J. Fungi 2021, 7(2), 81; https://doi.org/10.3390/jof7020081
Received: 24 December 2020 / Revised: 7 January 2021 / Accepted: 16 January 2021 / Published: 26 January 2021
(This article belongs to the Special Issue Candida auris 2.0)
Candida auris is a nosocomial pathogen responsible for an expanding global public health threat. This ascomycete yeast has been frequently isolated from hospital environments, representing a significant reservoir for transmission in healthcare settings. Here, we investigated the relationships among C. auris isolates from patients with chronic respiratory diseases admitted in a chest hospital and from their fomites, using whole-genome sequencing (WGS) and multilocus microsatellite genotyping. Overall, 37.5% (n = 12/32) patients developed colonisation by C. auris including 9.3% of the screened patients that were colonised at the time of admission and 75% remained colonised till discharge. Furthermore, 10% of fomite samples contained C. auris in rooms about 8.5 days after C. auris colonised patients were admitted. WGS and microsatellite typing revealed that multiple strains contaminated the fomites and colonised different body sites of patients. Notably, 37% of C. auris isolates were resistant to amphotericin B but with no amino acid substitution in ERG2, ERG3, ERG5, and ERG6 as compared to the reference strain B8441 in any of our strains. In addition, 55% of C. auris isolates likely had two copies of the MDR1 gene. Our results suggest significant genetic and ecological diversities of C. auris in healthcare setting. The WGS and microsatellite genotyping methods provided complementary results in genotype identification. View Full-Text
Keywords: Candida auris; colonisation; microsatellite typing; whole genome sequencing; ERG11; TAC1B; amphotericin B resistance in C. auris; India Candida auris; colonisation; microsatellite typing; whole genome sequencing; ERG11; TAC1B; amphotericin B resistance in C. auris; India
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MDPI and ACS Style

Yadav, A.; Singh, A.; Wang, Y.; Haren, M.H.v.; Singh, A.; de Groot, T.; Meis, J.F.; Xu, J.; Chowdhary, A. Colonisation and Transmission Dynamics of Candida auris among Chronic Respiratory Diseases Patients Hospitalised in a Chest Hospital, Delhi, India: A Comparative Analysis of Whole Genome Sequencing and Microsatellite Typing. J. Fungi 2021, 7, 81. https://doi.org/10.3390/jof7020081

AMA Style

Yadav A, Singh A, Wang Y, Haren MHv, Singh A, de Groot T, Meis JF, Xu J, Chowdhary A. Colonisation and Transmission Dynamics of Candida auris among Chronic Respiratory Diseases Patients Hospitalised in a Chest Hospital, Delhi, India: A Comparative Analysis of Whole Genome Sequencing and Microsatellite Typing. Journal of Fungi. 2021; 7(2):81. https://doi.org/10.3390/jof7020081

Chicago/Turabian Style

Yadav, Anamika, Anubhav Singh, Yue Wang, Merlijn H.v. Haren, Ashutosh Singh, Theun de Groot, Jacques F. Meis, Jianping Xu, and Anuradha Chowdhary. 2021. "Colonisation and Transmission Dynamics of Candida auris among Chronic Respiratory Diseases Patients Hospitalised in a Chest Hospital, Delhi, India: A Comparative Analysis of Whole Genome Sequencing and Microsatellite Typing" Journal of Fungi 7, no. 2: 81. https://doi.org/10.3390/jof7020081

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