Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains
2.2. Growth Conditions
2.3. Growth Curves and Cell Melanization
2.4. Immunofluorescence Assay
2.5. Laccase Activity
2.6. Phagocytosis Assay
2.7. Statistical Analysis
3. Results
3.1. Influence of L-DOPA on the Growth and Melanization of Yeasts in Liquid Medium among Paracoccidioides spp.
3.2. Analysis of Percentage of Melanization and Enzymatic Activities
3.3. Immunofluorescence Assay (IF)
3.4. Phagocytosis Assay
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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C. P. Emidio, E.; E. Urán J., M.; B. R. Silva, L.; S. Dias, L.; Doprado, M.; Nosanchuk, J.D.; Taborda, C.P. Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides. J. Fungi 2020, 6, 291. https://doi.org/10.3390/jof6040291
C. P. Emidio E, E. Urán J. M, B. R. Silva L, S. Dias L, Doprado M, Nosanchuk JD, Taborda CP. Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides. Journal of Fungi. 2020; 6(4):291. https://doi.org/10.3390/jof6040291
Chicago/Turabian StyleC. P. Emidio, Elúzia, Martha E. Urán J., Leandro B. R. Silva, Lucas S. Dias, Mariana Doprado, Joshua D. Nosanchuk, and Carlos Pelleschi Taborda. 2020. "Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides" Journal of Fungi 6, no. 4: 291. https://doi.org/10.3390/jof6040291