The Conserved MAP Kinase MpkB Regulates Development and Sporulation without Affecting Aflatoxin Biosynthesis in Aspergillus flavus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Construction of Deletion and Overexpression Mutants
2.3. Conidial and Sclerotial Production Analyses
2.4. Microscopy
2.5. Nucleic Acid Extraction and Northern Blot Analysis
2.6. Aflatoxin Extraction and Analysis via Thin-Layer Chromatography (TLC)
3. Results
3.1. Generation of A. flavus mpkB Deletion Strains
3.2. Deletion of mpkB Affects Conidiation and Sclerotia Production But Not Growth
3.3. Deletion of mpkB Caused Markedly Aberrant Conidiophore Morphology
3.4. Overexpression of mpkB Does Not Affect Growth and Development
3.5. MpkB Regulates brlA Expression during the Developmental Stage
3.6. MpkB Is Not Required for Aflatoxin Production in A. flavus
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Strain | Genotype | Use | Reference |
---|---|---|---|
NRRL3357 | Wild type | Wild-type strain | [40] |
NRRL3357-5 | pyrG− | Recipient strain | [40] |
SCWS1.02 | pyrG−, Afu_pyrG | pyrG complementation strain | This study |
SCWS1.11 | pyrG−, ΔmpkB:Afu_pyrG | mpkB deletion strain | This study |
SCWS1.20 | ΔmpkB::Afu_pyrG; mpkB, ptrA | mpkB complementation strain | This study |
SCWS1.35 | pyrG−; niiA(p)::mpkB, Afu_pyrG | mpkB overexpression strain | This study |
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Jun, S.-C.; Kim, J.-H.; Han, K.-H. The Conserved MAP Kinase MpkB Regulates Development and Sporulation without Affecting Aflatoxin Biosynthesis in Aspergillus flavus. J. Fungi 2020, 6, 289. https://doi.org/10.3390/jof6040289
Jun S-C, Kim J-H, Han K-H. The Conserved MAP Kinase MpkB Regulates Development and Sporulation without Affecting Aflatoxin Biosynthesis in Aspergillus flavus. Journal of Fungi. 2020; 6(4):289. https://doi.org/10.3390/jof6040289
Chicago/Turabian StyleJun, Sang-Cheol, Jong-Hwa Kim, and Kap-Hoon Han. 2020. "The Conserved MAP Kinase MpkB Regulates Development and Sporulation without Affecting Aflatoxin Biosynthesis in Aspergillus flavus" Journal of Fungi 6, no. 4: 289. https://doi.org/10.3390/jof6040289