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J. Fungi 2019, 5(2), 29; https://doi.org/10.3390/jof5020029

A Bright Future for Fluorescence Imaging of Fungi in Living Hosts

1
MRC Centre for Medical Mycology at the University of Aberdeen, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
2
Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
*
Author to whom correspondence should be addressed.
Received: 25 February 2019 / Revised: 27 March 2019 / Accepted: 29 March 2019 / Published: 3 April 2019
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Abstract

Traditional in vivo investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research. View Full-Text
Keywords: fluorescent reporters; bioluminescence; iRFP; live imaging; infection models; 3Rs fluorescent reporters; bioluminescence; iRFP; live imaging; infection models; 3Rs
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Chapuis, A.F.; Ballou, E.R.; MacCallum, D.M. A Bright Future for Fluorescence Imaging of Fungi in Living Hosts. J. Fungi 2019, 5, 29.

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