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Volume 9
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10.3390/vetsci9040193
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Article

The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of Brucella spp. in Greek Ruminants

by
Anthimia Batrinou
1,†,
Irini F. Strati
1,†,
Andreas G. Tsantes
2,†,
Joseph Papaparaskevas
3,†,
Ioannis Dimou
1,
Dimitrios Vourvidis
4,
Anna Kyrma
4,
Dionysis Antonopoulos
1,
Panagiotis Halvatsiotis
5 and
Dimitra Houhoula
1,*
1
Department of Food Science and Technology, University of West Attica, 12243 Egaleo, Greece
2
Microbiology Department, ‘Saint Savvas’ Oncology Hospital, 11522 Athens, Greece
3
Department of Microbiology, Medical School, National and Kapodistrian University of Athens, 15772 Athens, Greece
4
Laboratory of Brucellosis and Bacterial Disease Serology, Department of Diagnostic Pathology, Anatomy, Histology and Microbiology Directorate of Athens Veterinary Center, Ministry of Rural Development and Food, 11522 Athens, Greece
5
2nd Propaedeutic Department of Internal Medicine, Medical School, National and Kapodistrian University of Athens, “ATTIKON” University Hospital, Chaidari 12462, Greece
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Vet. Sci. 2022, 9(4), 193; https://doi.org/10.3390/vetsci9040193
Submission received: 16 March 2022 / Revised: 30 March 2022 / Accepted: 13 April 2022 / Published: 17 April 2022
(This article belongs to the Special Issue Diagnostic PCR on Animal Diseases: From Extraction to Amplification)
Review Reports Versions Notes

Abstract

The early and accurate diagnosis of brucellosis, a ubiquitous zoonotic infection, is significant in preventing disease transmission. This study aimed to assess the infection rate of Brucella spp. in ruminants and to evaluate the agreement between a serological test and a molecular method for the detection of infected cases. Blood and milk samples of 136 ruminants were analyzed using two laboratory methods: the Rose Bengal plate (RBP) test to detect B. abortus and B. melitensis antibodies and the molecular polymerase chain reaction (PCR) method for the presence of bacterial DNA. The agreement between the methods was assessed using the kappa statistic. Based on the RBP test, there were 12 (8.8%) seropositive animals (10 sheep and 2 cows), while 2 (1.4%) samples were positive on PCR analysis. The positive PCR samples were from seronegative cow samples on RBP testing. There was slight agreement (k = −0.02) between the two methods, which was not statistically significant. Our results indicate that complementary molecular methods are useful to detect the bacteria in infected animals that are seronegative due to an early stage of infection. Therefore, a combination of molecular methods and serological tests can be applied to detect brucellosis in ruminants efficiently.
Keywords: brucellosis; ruminants; PCR; antibodies; DNA; RBPT brucellosis; ruminants; PCR; antibodies; DNA; RBPT

Share and Cite

MDPI and ACS Style

Batrinou, A.; Strati, I.F.; Tsantes, A.G.; Papaparaskevas, J.; Dimou, I.; Vourvidis, D.; Kyrma, A.; Antonopoulos, D.; Halvatsiotis, P.; Houhoula, D. The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of Brucella spp. in Greek Ruminants. Vet. Sci. 2022, 9, 193. https://doi.org/10.3390/vetsci9040193

AMA Style

Batrinou A, Strati IF, Tsantes AG, Papaparaskevas J, Dimou I, Vourvidis D, Kyrma A, Antonopoulos D, Halvatsiotis P, Houhoula D. The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of Brucella spp. in Greek Ruminants. Veterinary Sciences. 2022; 9(4):193. https://doi.org/10.3390/vetsci9040193

Chicago/Turabian Style

Batrinou, Anthimia, Irini F. Strati, Andreas G. Tsantes, Joseph Papaparaskevas, Ioannis Dimou, Dimitrios Vourvidis, Anna Kyrma, Dionysis Antonopoulos, Panagiotis Halvatsiotis, and Dimitra Houhoula. 2022. "The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of Brucella spp. in Greek Ruminants" Veterinary Sciences 9, no. 4: 193. https://doi.org/10.3390/vetsci9040193

APA Style

Batrinou, A., Strati, I. F., Tsantes, A. G., Papaparaskevas, J., Dimou, I., Vourvidis, D., Kyrma, A., Antonopoulos, D., Halvatsiotis, P., & Houhoula, D. (2022). The Importance of Complementary PCR Analysis in Addition to Serological Testing for the Detection of Transmission Sources of Brucella spp. in Greek Ruminants. Veterinary Sciences, 9(4), 193. https://doi.org/10.3390/vetsci9040193

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