Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Granulosa Cell Isolation and Culture
2.2. Cell Treatment
2.3. Cell Viability Assay
2.4. Measurement of E2 Secretion
2.5. Gene Expression Analysis
2.6. Analysis and Detection of ROS
2.7. Analytical Oxidative Stress-Associated Parameters
2.8. Statistical Analysis
3. Results
3.1. ACT-A Enhances the Expression of FSHR and ERβ and Significantly Increases Granulosa Cells’ Sensitivity (GCs) Sensitivity to FSH Treatment
3.2. ACT-A Attenuates Apoptosis of GCs
3.3. ACT-A Mediates GCs Apoptosis via Modulating ERβ Expression
3.4. ACT-A Mediates Intracellular ROS Levels in GCs via ERβ
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Gene | Primer Sequences (5′—3′) | Length |
---|---|---|
β-Actin | F: CTTCCTGGGCATGGAGTCC | 201 bp |
R: GGCGCGATGATCTTGATCTTC | ||
CYP19A1 | F: GGTCACAACAAGACAGGA | 168 bp |
R: AACCAAGAGAAGAAAGCC | ||
FSHR | F: GCCCAGAACTAAAACACAATG | 107 bp |
R: TATAGACAAGTAACCGTCAGC | ||
BAX | F: AATTGGCTTGGTCTGTAT | 104 bp |
R: CGGTCGTGATGGTATGTG | ||
BCL2 | F: CATGCGTATTTATATTTG | 112 bp |
R: CTCTGCTGCTTGCTGCTA | ||
CASPASE 3 | F: ATGTCAGGCTAGTCTCTC | 124 bp |
R: TGGTATGTAACTTGGGGA | ||
ERβ | F: TATCTCCTCCCAGCAGCAGTCT | 153 bp |
F: TATCTCCTCCCAGCAGCAGTCT |
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Chen, F.; Zhu, X. Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation. Vet. Sci. 2022, 9, 704. https://doi.org/10.3390/vetsci9120704
Chen F, Zhu X. Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation. Veterinary Sciences. 2022; 9(12):704. https://doi.org/10.3390/vetsci9120704
Chicago/Turabian StyleChen, Fang, and Xiaoqing Zhu. 2022. "Activin A Reduces Porcine Granulosa Cells Apoptosis via ERβ-Dependent ROS Modulation" Veterinary Sciences 9, no. 12: 704. https://doi.org/10.3390/vetsci9120704