Explorative Field Study on the Use of Oral Fluids for the Surveillance of Actinobacillus pleuropneumoniae Infections in Fattening Farms by an Apx-Real-Time PCR
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Pigs/Farms
2.2. Data Collection
2.2.1. Sampling of OFs
2.2.2. Evaluation of Lung Lesions
2.2.3. Molecular Biological Examinations of OFs
2.3. Statistical Analysis
3. Results
3.1. OFs on Pen-Level at Different Times of Sampling
3.2. Lung Lesion Scoring at Slaughter
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Farm A | Farm B | |||||
---|---|---|---|---|---|---|
Time of Sampling | Pigs/Pen | Collected OFs/Pen | Total Collected | Pigs/Pen | Collected Ofs/Pen | Total Collected |
D 0–7 | 35 | 2 | 128 | 27 | 2 | 128 |
W 3 | 35 | 2 | 16 | 26–27 | 2 | 16 |
W 4 | 35 | 2 | 16 | - | - | - |
W 5–11 | 22–23 | 1 | 32 | 26–27 | 2 | 64 |
W 13 | 23 | 1 | 8 | 0–26 | 1–2 * | 14 |
W 15 | 8–21 | 1 | 8 | 1–15 | 1 * | 8 |
W17 | 0–8 | 0–1 * | 6 | - | - | - |
W 19 | 0–2 | 0–1 * | 4 | - | - | - |
Total | 218 | 230 |
Farm | Time | Apx IV–Apx III | Apx IV–Apx I |
---|---|---|---|
A | total | rs: 0.355, p < 0.001 | rs: 0.218, p = 0.001 |
W1 | - | rs: 0.377, p < 0.001 | |
W4 | rs: 0.746, p = 0.001 | - | |
W5 | - | rs: 0.764, p < 0.027 | |
B | Total | rs: 0.340, p < 0.001 | - |
W1 | rs: 0.364, p < 0.001 | - | |
W15 | rs: 0.741, p < 0.036 | - |
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Kleinmans, M.; Fiebig, K.; Tabeling, R.; Swam, H.; Duivelshof-Crienen, A.; Ritzmann, M.; Eddicks, M. Explorative Field Study on the Use of Oral Fluids for the Surveillance of Actinobacillus pleuropneumoniae Infections in Fattening Farms by an Apx-Real-Time PCR. Vet. Sci. 2022, 9, 552. https://doi.org/10.3390/vetsci9100552
Kleinmans M, Fiebig K, Tabeling R, Swam H, Duivelshof-Crienen A, Ritzmann M, Eddicks M. Explorative Field Study on the Use of Oral Fluids for the Surveillance of Actinobacillus pleuropneumoniae Infections in Fattening Farms by an Apx-Real-Time PCR. Veterinary Sciences. 2022; 9(10):552. https://doi.org/10.3390/vetsci9100552
Chicago/Turabian StyleKleinmans, Michael, Kerstin Fiebig, Robert Tabeling, Hanny Swam, Annelies Duivelshof-Crienen, Mathias Ritzmann, and Matthias Eddicks. 2022. "Explorative Field Study on the Use of Oral Fluids for the Surveillance of Actinobacillus pleuropneumoniae Infections in Fattening Farms by an Apx-Real-Time PCR" Veterinary Sciences 9, no. 10: 552. https://doi.org/10.3390/vetsci9100552