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Article

Swine Small Intestine Sealing Performed by Different Vessel Sealing Devices: Ex-Vivo Test

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Dipartimento dell’Emergenze e Trapianti di Organi (D.E.T.O.), Sezione di Cliniche Veterinarie e Produzioni Animali, Università degli Studi di Bari “Aldo Moro”, 70124 Bari, Italy
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Dottorato di Ricerca in “Trapianti di Tessuti ed Organi e Terapie Cellulari”, Dipartimento dell’Emergenza e Trapianti di Organi (D.E.T.O.), Università degli Studi di Bari “Aldo Moro”, 70124 Bari, Italy
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Dipartimento di Medicina Veterinaria, Sez. di Anatomia Patologica, Università degli Studi di Bari “Aldo Moro”, 70010 Bari, Italy
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Dipartimento di Scienze Mediche di Base, Neuroscienze e Organi di Senso, Università degli Studi di Bari “Aldo Moro”, 70124 Bari, Italy
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Author to whom correspondence should be addressed.
Academic Editors: Dominiek Maes and Kwonil Jung
Vet. Sci. 2021, 8(2), 34; https://doi.org/10.3390/vetsci8020034
Received: 8 February 2021 / Accepted: 19 February 2021 / Published: 22 February 2021
This study aimed to evaluate the sealing quality of swine small intestine using different laparoscopic radiofrequency vessel sealing devices (two 5 mm: RFVS-1 and -2; one 10 mm: RFVS-3) and a harmonic scalpel (HS) compared to golden standard closure technique. The study was divided into two arms. In study arm 1: n = 50 swine intestinal loops (10 per group) were transected with each instrument and the loops in which the devices provided complete sealing, at the gross inspection, were tested for maximum burst pressure (BP) and histological evaluation and compared to an automatic linear stapler. After the BP tests, the devices that achieved significantly lower BP values were excluded from the second arm. The RFVS-1 and -3 provided statistically significant results and were used in study arm 2, to obtain full-thickness biopsies along the antimesenteric border of the loop and were compared with hand-sewn intestinal closure (n = 30; 10 per group). The biopsies were histologically evaluated for thermal injury and diagnostic features, and intestinal loops tested for BP. RFVS-3 achieved comparable results (69.78 ± 4.23 mmHg, interquartile range (IQR) 5.8) to stapler closing technique (71.09 ± 4.22 mmHg, IQR 4.38; p > 0.05), while the RFVS-1 resulted in significantly (p < 0.05) lower BP (45.28 ± 15.23 mmHg, IQR 24.95) but over the physiological range, conversely to RFVS-2 (20.16 ± 7.19 mmHg, IQR 12.02) and HS (not measurable). RFVS-3 resulted not significantly different (p > 0.05) (45.09 ± 8.75 mmHg, IQR 10.48) than Suture (35.71 ± 17.51 mmHg, IQR 23.77); RFVS-1 resulted significantly lower values (23.96 ± 10.63 mmHg, IQR 9.62; p < 0.05). All biopsies were judged diagnostic. Data confirmed that RFVS-1 and -3 devices provided suitable intestinal sealing, with BP pressures over the physiological range. Conversely, the HS and RFVS-2 should not be considered for intestinal sealing. RFVS devices could be employed to obtain small intestine stump closure or full-thickness biopsies. However, further studies should be performed in live animals to assess the role of the healing process. View Full-Text
Keywords: small intestine; vessel sealing device; radiofrequency vessel sealing device; harmonic scalpel; full-thickness biopsy; burst pressure; leak pressure; swine small intestine; vessel sealing device; radiofrequency vessel sealing device; harmonic scalpel; full-thickness biopsy; burst pressure; leak pressure; swine
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MDPI and ACS Style

Lacitignola, L.; Imperante, A.; Trisciuzzi, R.; Zizzo, N.; Crovace, A.M.; Staffieri, F. Swine Small Intestine Sealing Performed by Different Vessel Sealing Devices: Ex-Vivo Test. Vet. Sci. 2021, 8, 34. https://doi.org/10.3390/vetsci8020034

AMA Style

Lacitignola L, Imperante A, Trisciuzzi R, Zizzo N, Crovace AM, Staffieri F. Swine Small Intestine Sealing Performed by Different Vessel Sealing Devices: Ex-Vivo Test. Veterinary Sciences. 2021; 8(2):34. https://doi.org/10.3390/vetsci8020034

Chicago/Turabian Style

Lacitignola, Luca, Annarita Imperante, Rodrigo Trisciuzzi, Nicola Zizzo, Alberto M. Crovace, and Francesco Staffieri. 2021. "Swine Small Intestine Sealing Performed by Different Vessel Sealing Devices: Ex-Vivo Test" Veterinary Sciences 8, no. 2: 34. https://doi.org/10.3390/vetsci8020034

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