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Open AccessArticle

Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against Staphylococcus aureus from Bovine Mastitis

by Isabel Titze 1 and Volker Krömker 1,2,*
1
Department of Bioprocess Engineering and Microbiology, Hannover University of Applied Sciences and Arts, D-30453 Hannover, Germany
2
University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary and Animal Sciences, Section for Production, Nutrition and Health, Gronnegardsvej 2, DK-1870 Frederiksberg C, Denmark
*
Author to whom correspondence should be addressed.
Vet. Sci. 2020, 7(1), 31; https://doi.org/10.3390/vetsci7010031
Received: 18 February 2020 / Revised: 2 March 2020 / Accepted: 4 March 2020 / Published: 6 March 2020
(This article belongs to the Section Clinical Veterinary Medicine)
The antimicrobial activity of a phage mixture and a lactic acid bacterium against Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1.ST29, EB1.ST11 and EB1.ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance. View Full-Text
Keywords: Staphylococcus aureus; bacteriophage mixture; phage therapy; lytic phage; lactic acid bacterium; dairy; bovine mastitis Staphylococcus aureus; bacteriophage mixture; phage therapy; lytic phage; lactic acid bacterium; dairy; bovine mastitis
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Titze, I.; Krömker, V. Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against Staphylococcus aureus from Bovine Mastitis. Vet. Sci. 2020, 7, 31.

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