Retrospective Investigation and Genetic Variation Analysis of Chicken Infectious Anemia in Shandong Province, 2020–2022
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection and Treatment
2.2. Viral DNA Detection by PCR
2.3. VP1 Gene Sequencing and Analysis
3. Results
3.1. PCR Results and Geographical Distribution of CAV-Positive Samples
3.2. Mixed Infections of Samples
3.3. Molecular Characterization of CAV VP1 Gene
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Primer Sequence (5′-3′) | Tm (°C) | Sizes (bp) |
---|---|---|---|
CAV-F | CAGAATTCCCACCTCAAGCGACTTCGAC | 55 | 582 |
CAV-R | ATGTCGACGGGGCTGAAGGAT | ||
FAdV-F | AATTTCGACCCCATGACGCGCCAGG | 56 | 508 |
FAdV-R | TGGCGAAAGGCGTACGGAAGTAAGC | ||
REV-F | CATACGAGCCAATGGTT | 54 | 300 |
REV-R | AATGTTGTAGCGAAGTACT | ||
ALV-J-F | GGATGAGGTGACTAAGA | 56 | 512 |
ALV-J-R | CGAACCAAAGGTAACACACG | ||
MDV-F | TCATCAGGGTCTCCCGTCACCT | 58 | 1005 |
MDV-R | AGAGATGTCTCAGGAGCCAGAG | ||
HEV-F1 | TCGCCT(C)GGTAAT(C)ACA(T)AATGC | 60 | 278 |
HEV-R1 | GCGTTC(G)CCG(C)ACAGGT(C)CGGCC | ||
HEV-F2 | ACA(T)AATGCT(C)AGGGTCACCCG | 56 | 242 |
HEV-R2 | ATGTACTGA(G)CCA(G)CTG(C)GCCGC |
Group | Strain | VP1 Amino Acid Site | Genotype | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
75 | 97 | 125 | 139 | 141 | 144 | 157 | 287 | 290 | 294 | |||
1 | on596886 | V | L | L | K | Q | E | M | N | P | H | A1 |
2 | KU645514 | · | M | · | · | · | · | V | · | · | · | A2 |
3 | DQ141673 | · | M | · | · | · | · | V | · | · | · | A3 |
4 | AF311470 | · | M | I | · | · | · | V | S | A | Q | B1 |
5 | MH001564 | I | · | I | Q | · | Q | V | A | · | Q | B2 |
6 | SDHY-210519 | · | · | · | · | · | · | · | T | · | · | A1 |
7 | SDHY-211016 | · | · | · | · | · | · | V | S | A | Q | A1 |
8 | SDHY-211126 | · | · | · | · | · | · | · | T | · | · | A1 |
9 | SDHY-211220 | · | · | · | · | · | · | · | T | · | · | A1 |
10 | SDHY-211224 | · | · | · | · | · | · | · | T | · | · | A1 |
11 | SDHY-220307 | · | · | · | · | · | · | · | S | · | · | A1 |
12 | SDHY-220616 | · | · | · | · | · | · | · | T | · | Q | A1 |
13 | SDHY-220725 | · | · | · | · | · | · | · | · | · | · | A1 |
14 | SDHY-220906 | · | · | · | · | · | · | · | · | · | Q | A1 |
15 | SDHY-220907 | · | · | · | · | · | · | · | · | · | · | A1 |
16 | SDHY-220912 | · | · | · | · | · | · | · | · | · | · | A1 |
17 | SDHY-220917 | · | · | · | · | · | · | · | · | · | · | A1 |
18 | SDHY-220801 | · | M | · | · | · | · | · | S | A | Q | A2 |
19 | SDHY-210825 | · | · | · | · | · | · | V | S | A | Q | A3 |
20 | SDHY-220519 | I | · | I | Q | · | Q | V | A | A | Q | B2 |
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Li, J.; Lou, Y.; Li, P.; Wang, T.; Lv, Z.; Guo, Z.; Geng, N.; Meng, F.; Liu, S.; Li, N. Retrospective Investigation and Genetic Variation Analysis of Chicken Infectious Anemia in Shandong Province, 2020–2022. Vet. Sci. 2023, 10, 263. https://doi.org/10.3390/vetsci10040263
Li J, Lou Y, Li P, Wang T, Lv Z, Guo Z, Geng N, Meng F, Liu S, Li N. Retrospective Investigation and Genetic Variation Analysis of Chicken Infectious Anemia in Shandong Province, 2020–2022. Veterinary Sciences. 2023; 10(4):263. https://doi.org/10.3390/vetsci10040263
Chicago/Turabian StyleLi, Jing, Yufei Lou, Peixun Li, Tailong Wang, Zehao Lv, Zhiyun Guo, Ningwei Geng, Fanliang Meng, Sidang Liu, and Ning Li. 2023. "Retrospective Investigation and Genetic Variation Analysis of Chicken Infectious Anemia in Shandong Province, 2020–2022" Veterinary Sciences 10, no. 4: 263. https://doi.org/10.3390/vetsci10040263