The Influence of Green and Black Tea Infusion Parameters on Total Polyphenol Content and Antioxidant Activity by ABTS and DPPH Assays
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Instruments
2.3. Samples
2.4. Aqueous Tea Extract Preparation
2.5. Polyphenols Aqueous Extraction at Different pH
2.6. Determination of Total Polyphenol Content (Folin–Ciocâlteu)
2.7. Determination of Antioxidant Activity (ABTS and DPPH Assays)
2.8. Statistical Analysis
3. Results and Discussion
3.1. Effect of Infusion Variables on TPC
3.2. Effect of pH of Aqueous Solution on TPC
3.3. ABTS Assay of Tea Infusions
3.4. DPPH Activity of Tea Infusions
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Parameter | Unit | NMW | TP | DW |
---|---|---|---|---|
Electrical conductivity at 20 °C | µS/cm | 668 | 571 | 6.8 |
pH | - | 7.06 | 7.50 | 7.00 |
Fixed residue | mg/L | 440 | 408 | <1 |
Hardness | F° | 13.3 | 33.5 | <0.01 |
Calcium (Ca2+) | mg/L | 124 | 104.0 | - |
Magnesium (Mg+) | mg/L | 29.4 | 18.70 | - |
Sodium (Na+) | mg/L | 4.0 | 4.1 | - |
Potassium (K+) | mg/L | 1.2 | 0.97 | - |
Bicarbonate (HCO3−) | mg/L | 498 | 399 | - |
Sulfates (SO42−) | mg/L | 17.2 | 16.60 | - |
Chlorides (Cl−) | mg/L | 6.6 | 6.5 | - |
Nitrates (NO3−) | mg/L | 2 | 2.99 | - |
Nitrites (NO2−) | mg/L | <0.002 | <0.01 | - |
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Vinci, G.; D’Ascenzo, F.; Maddaloni, L.; Prencipe, S.A.; Tiradritti, M. The Influence of Green and Black Tea Infusion Parameters on Total Polyphenol Content and Antioxidant Activity by ABTS and DPPH Assays. Beverages 2022, 8, 18. https://doi.org/10.3390/beverages8020018
Vinci G, D’Ascenzo F, Maddaloni L, Prencipe SA, Tiradritti M. The Influence of Green and Black Tea Infusion Parameters on Total Polyphenol Content and Antioxidant Activity by ABTS and DPPH Assays. Beverages. 2022; 8(2):18. https://doi.org/10.3390/beverages8020018
Chicago/Turabian StyleVinci, Giuliana, Fabrizio D’Ascenzo, Lucia Maddaloni, Sabrina Antonia Prencipe, and Margherita Tiradritti. 2022. "The Influence of Green and Black Tea Infusion Parameters on Total Polyphenol Content and Antioxidant Activity by ABTS and DPPH Assays" Beverages 8, no. 2: 18. https://doi.org/10.3390/beverages8020018