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Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors

1
Department of Pediatrics, University of Florida, Gainesville, FL 32603, USA
2
Department of Neurology, University of Minnesota, Minneapolis, MN 55455, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Denise Salzig
Bioengineering 2021, 8(7), 96; https://doi.org/10.3390/bioengineering8070096
Received: 2 May 2021 / Revised: 2 July 2021 / Accepted: 2 July 2021 / Published: 7 July 2021
Human mesenchymal stem cells (hMSCs) have demonstrated great potential to be used as therapies for many types of diseases. Due to their immunoprivileged status, allogeneic hMSCs therapies are particularly attractive options and methodologies to improve their scaling and manufacturing are needed. Microcarrier-based bioreactor systems provide higher volumetric hMSC production in automated closed systems than conventional planar cultures. However, more sophisticated bioprocesses are necessary to successfully convert from planar culture to microcarriers. This article summarizes key steps involved in the planar culture to microcarrier hMSC manufacturing scheme, from seed train, inoculation, expansion and harvest. Important bioreactor parameters, such as temperature, pH, dissolved oxygen (DO), mixing, feeding strategies and cell counting techniques, are also discussed. View Full-Text
Keywords: human mesenchymal stem cell; hMSC; bioprocess; bioreactor; microcarrier; cell expansion; cell counting human mesenchymal stem cell; hMSC; bioprocess; bioreactor; microcarrier; cell expansion; cell counting
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MDPI and ACS Style

Tsai, A.-C.; Pacak, C.A. Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors. Bioengineering 2021, 8, 96. https://doi.org/10.3390/bioengineering8070096

AMA Style

Tsai A-C, Pacak CA. Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors. Bioengineering. 2021; 8(7):96. https://doi.org/10.3390/bioengineering8070096

Chicago/Turabian Style

Tsai, Ang-Chen, and Christina A. Pacak. 2021. "Bioprocessing of Human Mesenchymal Stem Cells: From Planar Culture to Microcarrier-Based Bioreactors" Bioengineering 8, no. 7: 96. https://doi.org/10.3390/bioengineering8070096

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