3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Nanocellulose
2.2. Atomic Force Microscopy and Nano-Mechanical Assessment
2.3. 3D Printing
2.4. 2D Cell Culture
2.5. 3D Cell Culture
2.6. Cell Viability Assessment
2.7. Gene Expression Analysis
2.8. Scanning Electron Microscopy (SEM)
2.9. Hematoxylin and Eosin Staining
2.10. Holoclone Assay
2.11. Statistics
3. Results
3.1. Nanocellulose Characteristics
3.2. Printability Evaluation
3.3. 3D Printed CNF Suitable as Cell Growth Scaffolds
3.4. 3D CNF Environment Induce Stem Cell Properties
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rosendahl, J.; Svanström, A.; Berglin, M.; Petronis, S.; Bogestål, Y.; Stenlund, P.; Standoft, S.; Ståhlberg, A.; Landberg, G.; Chinga-Carrasco, G.; et al. 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System. Bioengineering 2021, 8, 97. https://doi.org/10.3390/bioengineering8070097
Rosendahl J, Svanström A, Berglin M, Petronis S, Bogestål Y, Stenlund P, Standoft S, Ståhlberg A, Landberg G, Chinga-Carrasco G, et al. 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System. Bioengineering. 2021; 8(7):97. https://doi.org/10.3390/bioengineering8070097
Chicago/Turabian StyleRosendahl, Jennifer, Andreas Svanström, Mattias Berglin, Sarunas Petronis, Yalda Bogestål, Patrik Stenlund, Simon Standoft, Anders Ståhlberg, Göran Landberg, Gary Chinga-Carrasco, and et al. 2021. "3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System" Bioengineering 8, no. 7: 97. https://doi.org/10.3390/bioengineering8070097