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Challenges and Solutions for Commercial Scale Manufacturing of Allogeneic Pluripotent Stem Cell Products
Review

Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications

iBB-Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
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Bioengineering 2020, 7(2), 36; https://doi.org/10.3390/bioengineering7020036
Received: 14 March 2020 / Revised: 3 April 2020 / Accepted: 7 April 2020 / Published: 9 April 2020
(This article belongs to the Special Issue Stem Cell Bioprocessing and Manufacturing)
Liver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has been limited due to the lack of in vitro models that mimic in vivo liver physiology. Accordingly, hepatic cell lineages derived from human pluripotent stem cells (hPSCs) represent a promising cell source for liver cell therapy, disease modelling, and drug discovery. Moreover, the development of new culture systems bringing together the multiple liver-specific hepatic cell types triggered the development of hPSC-derived liver organoids. Therefore, these human liver-based platforms hold great potential for clinical applications. In this review, the production of the different hepatic cell lineages from hPSCs, including hepatocytes, as well as the emerging strategies to generate hPSC-derived liver organoids will be assessed, while current biomedical applications will be highlighted. View Full-Text
Keywords: human pluripotent stem cells; hepatic cell lineages; hepatocyte differentiation; non-parenchymal liver cells; liver organoids; disease modeling; drug screening human pluripotent stem cells; hepatic cell lineages; hepatocyte differentiation; non-parenchymal liver cells; liver organoids; disease modeling; drug screening
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MDPI and ACS Style

Cotovio, J.P.; Fernandes, T.G. Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications. Bioengineering 2020, 7, 36. https://doi.org/10.3390/bioengineering7020036

AMA Style

Cotovio JP, Fernandes TG. Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications. Bioengineering. 2020; 7(2):36. https://doi.org/10.3390/bioengineering7020036

Chicago/Turabian Style

Cotovio, João P., and Tiago G. Fernandes. 2020. "Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications" Bioengineering 7, no. 2: 36. https://doi.org/10.3390/bioengineering7020036

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