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Bioengineering 2019, 6(1), 3; https://doi.org/10.3390/bioengineering6010003

Optimization of Decellularization Procedure in Rat Esophagus for Possible Development of a Tissue Engineered Construct

1
Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece
2
Center of Experimental Surgery, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, Greece
These authors were equally contributed to this work as first authors.
*
Author to whom correspondence should be addressed.
Received: 5 December 2018 / Revised: 19 December 2018 / Accepted: 20 December 2018 / Published: 24 December 2018
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
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Abstract

Background: Current esophageal treatment is associated with significant morbidity. The gold standard therapeutic strategies are stomach interposition or autografts derived from the jejunum and colon. However, severe adverse reactions, such as esophageal leakage, stenosis and infection, accompany the above treatments, which, most times, are life threating. The aim of this study was the optimization of a decellularization protocol in order to develop a proper esophageal tissue engineered construct. Methods: Rat esophagi were obtained from animals and were decellularized. The decellularization process involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) buffers for 6 h each, followed by incubation in a serum medium. The whole process involved two decellularization cycles. Then, a histological analysis was performed. In addition, the amounts of collagen, sulphated glycosaminoglycans and DNA content were quantified. Results: The histological analysis revealed that only the first decellularization cycle was enough to produce a cellular and nuclei free esophageal scaffold with a proper extracellular matrix orientation. These results were further confirmed by biochemical quantification. Conclusions: Based on the above results, the current decellularization protocol can be applied successfully in order to produce an esophageal tissue engineered construct. View Full-Text
Keywords: esophagus; Barret’s esophagus; decellularization; CHAPS; SDS; histological images; tissue engineered construct esophagus; Barret’s esophagus; decellularization; CHAPS; SDS; histological images; tissue engineered construct
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Mallis, P.; Chachlaki, P.; Katsimpoulas, M.; Stavropoulos-Giokas, C.; Michalopoulos, E. Optimization of Decellularization Procedure in Rat Esophagus for Possible Development of a Tissue Engineered Construct. Bioengineering 2019, 6, 3.

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