Associations of Ambient Environmental Conditions with Growth and Dissemination of Staphylococcus epidermidis on the Surface of Teatcups from Sheep Milking Parlours
Abstract
:1. Introduction
2. Materials and Methods
2.1. Staphylococcal Isolates
2.2. Test Material
2.3. Experimental Work
2.4. Ambient Conditions
2.5. Data Management and Analysis
3. Results
3.1. Frequency of Staphylococcal Recoveries
3.2. Speed of Staphylococcal Dissemination
3.3. Teatcup Surface Covered by Staphylococci
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Time after Application by Smearing | Total Recoveries | ||
---|---|---|---|
New Teatcups | Used Teatcups | p | |
Humidity 60% | |||
3 h | 1/144 | 0/144 | 0.32 |
6 h | 58/144 | 2/144 | <0.0001 |
9 h | 109/144 | 30/144 | <0.0001 |
12 h | 144/144 | 86/144 | <0.0001 |
15 h | 144/144 | 132/144 | 0.0004 |
18 h | 144/144 | 144/144 | n/a |
24 h | 144/144 | 144/144 | n/a |
Total | 744/1008 | 538/1008 | <0.0001 |
Humidity 80% | |||
3 h | 5/144 | 10/144 | 0.18 |
6 h | 124/144 | 100/144 | 0.0007 |
9 h | 144/144 | 143/144 | 0.32 |
12 h | 144/144 | 144/144 | n/a |
15 h | 144/144 | 144/144 | n/a |
18 h | 144/144 | 144/144 | n/a |
24 h | 144/144 | 144/144 | n/a |
Total | 849/1008 | 829/1008 | 0.23 |
Grand total | 1593/2016 | 1367/2016 | <0.0001 |
Time after Application by Smearing | Total Recoveries | ||
---|---|---|---|
New Teatcups | Used Teatcups | p | |
Temperature 21 °C | |||
3 h | 1/144 | 3/144 | 0.56 |
6 h | 82/144 | 46/144 | <0.0001 |
9 h | 122/144 | 81/144 | <0.0001 |
12 h | 144/144 | 104/144 | <0.0001 |
15 h | 144/144 | 132/144 | 0.0004 |
18 h | 144/144 | 144/144 | n/a |
24 h | 144/144 | 144/144 | n/a |
Total | 781/1008 | 654/1008 | <0.0001 |
Temperature 31 °C | |||
3 h | 5/144 | 7/144 | 0.56 |
6 h | 100/144 | 56/144 | <0.0001 |
9 h | 131/144 | 92/144 | <0.0001 |
12 h | 144/144 | 126/144 | <0.0001 |
15 h | 144/144 | 144/144 | n/a |
18 h | 144/144 | 144/144 | n/a |
24 h | 144/144 | 144/144 | n/a |
Total | 812/1008 | 713/1008 | <0.0001 |
Grand total | 1593/2016 | 1367/2016 | <0.0001 |
Ambient Conditions 1 | New Teatcups | Used Teatcups | p |
---|---|---|---|
21 °C, 60% | 5.048 ± 0.930 a,b | 2.588 ± 0.459 a,b | 0.039 |
21 °C, 80% | 17.170 ± 3.953 a,c | 20.119 ± 3.070 a,c | 0.57 |
31 °C, 60% | 9.682 ± 1.061 d | 5.145 ± 0.566 c,d | 0.004 |
31 °C, 80% | 27.730 ± 2.460 b,c,d | 25.491 ± 3.271 b,d | 0.60 |
p | <0.0001 | <0.0001 |
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Katsarou, E.I.; Petinaki, E.; Fthenakis, G.C. Associations of Ambient Environmental Conditions with Growth and Dissemination of Staphylococcus epidermidis on the Surface of Teatcups from Sheep Milking Parlours. Bioengineering 2023, 10, 81. https://doi.org/10.3390/bioengineering10010081
Katsarou EI, Petinaki E, Fthenakis GC. Associations of Ambient Environmental Conditions with Growth and Dissemination of Staphylococcus epidermidis on the Surface of Teatcups from Sheep Milking Parlours. Bioengineering. 2023; 10(1):81. https://doi.org/10.3390/bioengineering10010081
Chicago/Turabian StyleKatsarou, Eleni I., Efthymia Petinaki, and George C. Fthenakis. 2023. "Associations of Ambient Environmental Conditions with Growth and Dissemination of Staphylococcus epidermidis on the Surface of Teatcups from Sheep Milking Parlours" Bioengineering 10, no. 1: 81. https://doi.org/10.3390/bioengineering10010081
APA StyleKatsarou, E. I., Petinaki, E., & Fthenakis, G. C. (2023). Associations of Ambient Environmental Conditions with Growth and Dissemination of Staphylococcus epidermidis on the Surface of Teatcups from Sheep Milking Parlours. Bioengineering, 10(1), 81. https://doi.org/10.3390/bioengineering10010081