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Effective Concentration of Ionic Liquids for Enhanced Saccharification of Cellulose

Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1–3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
Hitachi Zosen Corporation, 1-7-89 Nanko-kita, Sumiyoshi-ku, Osaka 559-8559, Japan
Author to whom correspondence should be addressed.
ChemEngineering 2018, 2(4), 47;
Received: 20 July 2018 / Revised: 22 August 2018 / Accepted: 27 September 2018 / Published: 3 October 2018
PDF [898 KB, uploaded 4 October 2018]


In an aqueous enzymatic saccharification using cellulase, the dissolution of crystalline cellulose is one of the rate-limiting steps. Insoluble cellulose powder was preliminarily heat-treated with ionic liquids (ILs), such as [Bmim][Cl] (1-butyl-3-methylimidazolium chloride) and [Amim][Cl] (1-allyl-3-methylimidazolium chloride), which enable the production of soluble cellulose. On the other hand, the presence of ILs leads to a denaturation of enzymes. Using cellulase from Trichoderma viride, the effects of [Bmim][Cl] and [Amim][Cl] in the enzymatic saccharification were compared. The production of glucose was optimized with 5 wt%-ILs, both for [Bmim][Cl] and for [Amim][Cl]. The significant inhibiting effects of ILs (IL concentration >10 wt%) could be due to the denaturation of cellulase, because the peak shifts of intrinsic tryptophan fluorescence were observed in the presence of 7.5 wt%-ILs. To analyze kinetic parameters, the Langmuir adsorption model and the Michaelis-Menten model were employed. The investigation suggests that [Amim][Cl] can provide soluble cellulose more efficiently, and can promote enzymatic saccharification in the IL concentration below 5 wt%. View Full-Text
Keywords: ionic liquid; cellulase; insoluble cellulose; hydrolysis; kinetic analysis ionic liquid; cellulase; insoluble cellulose; hydrolysis; kinetic analysis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tanimura, K.; Ooe, Y.; Suga, K.; Umakoshi, H. Effective Concentration of Ionic Liquids for Enhanced Saccharification of Cellulose. ChemEngineering 2018, 2, 47.

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