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Open AccessArticle

Effective Concentration of Ionic Liquids for Enhanced Saccharification of Cellulose

1
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1–3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan
2
Hitachi Zosen Corporation, 1-7-89 Nanko-kita, Sumiyoshi-ku, Osaka 559-8559, Japan
*
Author to whom correspondence should be addressed.
ChemEngineering 2018, 2(4), 47; https://doi.org/10.3390/chemengineering2040047
Received: 20 July 2018 / Revised: 22 August 2018 / Accepted: 27 September 2018 / Published: 3 October 2018
In an aqueous enzymatic saccharification using cellulase, the dissolution of crystalline cellulose is one of the rate-limiting steps. Insoluble cellulose powder was preliminarily heat-treated with ionic liquids (ILs), such as [Bmim][Cl] (1-butyl-3-methylimidazolium chloride) and [Amim][Cl] (1-allyl-3-methylimidazolium chloride), which enable the production of soluble cellulose. On the other hand, the presence of ILs leads to a denaturation of enzymes. Using cellulase from Trichoderma viride, the effects of [Bmim][Cl] and [Amim][Cl] in the enzymatic saccharification were compared. The production of glucose was optimized with 5 wt%-ILs, both for [Bmim][Cl] and for [Amim][Cl]. The significant inhibiting effects of ILs (IL concentration >10 wt%) could be due to the denaturation of cellulase, because the peak shifts of intrinsic tryptophan fluorescence were observed in the presence of 7.5 wt%-ILs. To analyze kinetic parameters, the Langmuir adsorption model and the Michaelis-Menten model were employed. The investigation suggests that [Amim][Cl] can provide soluble cellulose more efficiently, and can promote enzymatic saccharification in the IL concentration below 5 wt%. View Full-Text
Keywords: ionic liquid; cellulase; insoluble cellulose; hydrolysis; kinetic analysis ionic liquid; cellulase; insoluble cellulose; hydrolysis; kinetic analysis
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MDPI and ACS Style

Tanimura, K.; Ooe, Y.; Suga, K.; Umakoshi, H. Effective Concentration of Ionic Liquids for Enhanced Saccharification of Cellulose. ChemEngineering 2018, 2, 47.

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