Next Article in Journal
Environmentally Relevant Concentrations of Bisphenol A Interact with Doxorubicin Transcriptional Effects in Human Cell Lines
Next Article in Special Issue
Toxicity of Aqueous L-Selenomethionine and Tert-Butyl Hydroperoxide Exposure to Zebrafish (Danio rerio) Embryos Following Tert-Butyl Hydroquinone Treatment
Previous Article in Journal
Towards Mass Spectrometry-Based Chemical Exposome: Current Approaches, Challenges, and Future Directions
Previous Article in Special Issue
Cytotoxicity and Toxicity Evaluation of Xanthone Crude Extract on Hypoxic Human Hepatocellular Carcinoma and Zebrafish (Danio rerio) Embryos
Open AccessArticle

Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio)

Department of Biological Science, University of Calgary, Calgary, AB T2N 1N4, Canada
Department of Civil Engineering, University of Calgary, Calgary, AB T2N 1N4, Canada
Author to whom correspondence should be addressed.
Toxics 2019, 7(3), 42;
Received: 13 June 2019 / Revised: 20 August 2019 / Accepted: 20 August 2019 / Published: 23 August 2019
(This article belongs to the Special Issue Contaminant Effects on Zebrafish Embryos)
Sulfolane is a widely used polar, aprotic solvent that has been detected by chemical analysis in groundwater and creeks around the world including Alberta, Canada (800 µg/mL), Louisiana, USA (2900 µg/mL) and Brisbane, Australia (4344 µg/mL). Previous research provided information on adverse effects of sulfolane on mammals, but relatively little information is available on aquatic organisms. This study tested the effects of sulfolane (0–5000 µg/mL) on early development of zebrafish larvae, using various morphometric (survival, hatching, yolk sac and pericardial oedema, haemorrhaging, spinal malformations, swim bladder inflation), growth (larval length, eye volume, yolk sac utilisation), behavioural (touch response, locomotor activity and transcript abundance parameters (ahr1a, cyp1a, thraa, dio1, dio2, dio3, 11βhsd2, gr, aqp3a, cyp19a1b, ddc, gria2b and hsp70) for 120 h. Embryos were chronically exposed to sulfolane throughout the experimental period. For locomotor activity, however, we also investigated acute response to 2-h sulfolane treatment. Sulfolane sensitivity causing significant impairment in the observed parameters were different depending on parameters measured, including survival (concentrations greater than 800 µg/mL), morphometric and growth (800–1000 µg/mL), behaviour (500–800 µg/mL) and transcript abundance (10 µg/mL). The overall results provide novel information on the adverse health impacts of sulfolane on an aquatic vertebrate species, and an insight into developmental impairments following exposure to environmental levels of sulfolane in fish embryos. View Full-Text
Keywords: sulfolane; toxicity; morphometrics; behaviour; gene expression; zebrafish sulfolane; toxicity; morphometrics; behaviour; gene expression; zebrafish
Show Figures

Figure 1

MDPI and ACS Style

Shah, S.M.; Wahba, M.; Yu, L.; Achari, G.; Habibi, H.R. Health Impact Assessment of Sulfolane on Embryonic Development of Zebrafish (Danio rerio). Toxics 2019, 7, 42.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop