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Article

A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain

1
Istituto Italiano di Tecnologia, Nanobiointeractions & Nanodiagnostics, Via Morego 30, 16163 Genova, Italy
2
Department of Chemistry and Industrial Chemistry, University of Genova, Via Dodecaneso 31, 16146 Genova, Italy
3
Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics, Via San Protaso 302, 29017 Fiorenzuola d’Arda PC, Italy
4
Barilla S.p.A., Via Mantova 166, 43122 Parma PR, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Foods 2020, 9(11), 1691; https://doi.org/10.3390/foods9111691
Received: 25 September 2020 / Revised: 5 November 2020 / Accepted: 13 November 2020 / Published: 19 November 2020
(This article belongs to the Section Food Analytical Methods)
The development of a colorimetric mono-varietal discriminating assay, aimed at improving traceability and quality control checks of durum wheat products, is described. A single nucleotide polymorphism (SNP) was identified as a reliable marker for wheat varietal discrimination, and a rapid test for easy and clear identification of specific wheat varieties was developed. Notably, an approach based on the loop-mediated isothermal amplification reaction (LAMP) as an SNP discrimination tool, in combination with naked-eye visualization of the results, was designed and optimized. Our assay was proven to be effective in the detection of adulterated food products, including both substitution and mixing with different crop varieties. View Full-Text
Keywords: durum wheat variety; genetic traceability; single nucleotide polymorphisms; colorimetric tests; authenticity; point of care test durum wheat variety; genetic traceability; single nucleotide polymorphisms; colorimetric tests; authenticity; point of care test
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MDPI and ACS Style

Cibecchini, G.; Cecere, P.; Tumino, G.; Morcia, C.; Ghizzoni, R.; Carnevali, P.; Terzi, V.; Pompa, P.P. A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain. Foods 2020, 9, 1691. https://doi.org/10.3390/foods9111691

AMA Style

Cibecchini G, Cecere P, Tumino G, Morcia C, Ghizzoni R, Carnevali P, Terzi V, Pompa PP. A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain. Foods. 2020; 9(11):1691. https://doi.org/10.3390/foods9111691

Chicago/Turabian Style

Cibecchini, Giulia; Cecere, Paola; Tumino, Giorgio; Morcia, Caterina; Ghizzoni, Roberta; Carnevali, Paola; Terzi, Valeria; Pompa, Pier P. 2020. "A Fast, Naked-Eye Assay for Varietal Traceability in the Durum Wheat Production Chain" Foods 9, no. 11: 1691. https://doi.org/10.3390/foods9111691

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