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Article

Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells

1
Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Via Galeazzi 4, 20161 Milan, Italy
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Department of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133 Milan, Italy
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Unitech COSPECT—University Technological Platforms Office, University of Milan, Via Golgi 19, 20133 Milan, Italy
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Department of Athletics, Strength and Conditioning, Poznań University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań, Poland
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Vita-Salute San Raffaele University, Via Olgettina 58, 20132 Milan, Italy
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Department of Biomedical Sciences for Health, University of Milan, Via Mangiagalli 31, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Foods 2020, 9(10), 1510; https://doi.org/10.3390/foods9101510
Received: 21 September 2020 / Revised: 13 October 2020 / Accepted: 19 October 2020 / Published: 21 October 2020
(This article belongs to the Section Food Nutrition)
Infant biscuits (IBs) are part of complementary feeding from weaning up to the age of five years. They normally contain bovine milk proteins, which can influence bone development. This potential effect was investigated using experimental baked IBs, which were prepared from doughs containing different type of dairy proteins: milk protein concentrate (IB1), whey protein isolate (IB2), and skimmed milk powder (IB3). Dairy protein-free (IB0) and gluten-free (IB4) biscuits were also formulated. The in vitro gastrointestinal digests of IBs (IBDs) were tested on a co-culture of Caco-2/HT-29 70/30 cells as an in vitro model of human small intestine. None of the IBDs influenced cell viability and monolayer integrity, while IBD0 and IBD4 increased Peptide-YY production. The basolateral contents of Transwell plates seeded with Caco-2/HT-29 70/30 co-culture, mimicking metabolized IBDs (MIBDs), were tested on Saos-2 cells, an in vitro model of human osteoblast-like cells. After incubation, MIBD0, lacking dairy proteins, decreased the cell viability, while MIBD2, containing whey protein isolate, increased both the viability and the number of cells. MIBD2 and MIBD4, the latter containing both casein and whey proteins, increased alkaline phosphatase activity, a bone differentiation marker. These results highlight that IBs containing dairy proteins positively affect bone development. View Full-Text
Keywords: infant biscuits; dairy proteins; whey; casein; Caco-2/HT-29 co-culture cells; Saos-2 cells; bone infant biscuits; dairy proteins; whey; casein; Caco-2/HT-29 co-culture cells; Saos-2 cells; bone
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MDPI and ACS Style

Bottani, M.; Cattaneo, S.; Pica, V.; Stuknytė, M.; Gomarasca, M.; Lombardi, G.; Banfi, G.; Noni, I.D.; Ferraretto, A. Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells. Foods 2020, 9, 1510. https://doi.org/10.3390/foods9101510

AMA Style

Bottani M, Cattaneo S, Pica V, Stuknytė M, Gomarasca M, Lombardi G, Banfi G, Noni ID, Ferraretto A. Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells. Foods. 2020; 9(10):1510. https://doi.org/10.3390/foods9101510

Chicago/Turabian Style

Bottani, Michela, Stefano Cattaneo, Valentina Pica, Milda Stuknytė, Marta Gomarasca, Giovanni Lombardi, Giuseppe Banfi, Ivano D. Noni, and Anita Ferraretto. 2020. "Gastrointestinal In Vitro Digests of Infant Biscuits Formulated with Bovine Milk Proteins Positively Affect In Vitro Differentiation of Human Osteoblast-Like Cells" Foods 9, no. 10: 1510. https://doi.org/10.3390/foods9101510

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