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Open AccessArticle

Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source

1
Department of Animal Science, Division of Microbiology and Animal Hygiene, Georg-August-University of Goettingen, D-37077, Goettingen, Germany
2
Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, D-04103, Leipzig, Germany
*
Author to whom correspondence should be addressed.
Foods 2020, 9(10), 1392; https://doi.org/10.3390/foods9101392
Received: 30 July 2020 / Revised: 18 September 2020 / Accepted: 28 September 2020 / Published: 1 October 2020
(This article belongs to the Section Food Quality and Safety)
Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established a rapid sequencing protocol to identify animal species within hours. Sequencing was achieved by nanopore sequencing and data analysis via offline BLAST search. The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1% of horse, chicken, turkey, cattle, sheep, duck, rabbit, goat, and donkey. The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products. View Full-Text
Keywords: meat adulteration; rapid sequencing protocol; nanopore sequencing; point-of-need meat adulteration; rapid sequencing protocol; nanopore sequencing; point-of-need
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MDPI and ACS Style

Kissenkötter, J.; Böhlken-Fascher, S.; Abd El Wahed, A. Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source. Foods 2020, 9, 1392. https://doi.org/10.3390/foods9101392

AMA Style

Kissenkötter J, Böhlken-Fascher S, Abd El Wahed A. Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source. Foods. 2020; 9(10):1392. https://doi.org/10.3390/foods9101392

Chicago/Turabian Style

Kissenkötter, Jonas; Böhlken-Fascher, Susanne; Abd El Wahed, Ahmed. 2020. "Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source" Foods 9, no. 10: 1392. https://doi.org/10.3390/foods9101392

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