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In Vitro Production and Identification of Angiotensin Converting Enzyme (ACE) Inhibitory Peptides Derived from Distilled Spent Grain Prolamin Isolate

Key Laboratory of Industrial Biotechnology of Ministry of Education, Laboratory of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Jiangnan University, Wuxi 214000, Jiangsu, China
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Foods 2019, 8(9), 390; https://doi.org/10.3390/foods8090390
Received: 16 August 2019 / Revised: 28 August 2019 / Accepted: 28 August 2019 / Published: 4 September 2019
(This article belongs to the Section Nutraceuticals and Functional Foods)
Distilled spent grain (DSG), the biggest by-product of the Chinese liquor industry, is rich in protein (167.8 g/kg DSG dry weight (DW)). Accounting for 60% of the total protein, prolamins are isolated from dried DSG (DDSG). In this study, angiotensin-converting enzyme (ACE) inhibitory peptides were screened from the prolamin hydrolysates of DDSG using two independent active-directed separations, ultrafiltration and reversed phase high performance liquid chromatography (RP-HPLC) coupled with ACE inhibitory activity evaluation. Six novel ACE inhibitory peptides, AVQ, YPQ, NQL, AYLQ, VLPVLS, and VLPSLN, were successfully identified and quantified from the active RP-HPLC fractions. AVQ and YPQ exhibited the highest activity, having the concentration inducing 50% inhibition (IC50) values for ACE of 181.0 and 220.0 μM, respectively. It was observed that VLPVLS was the most abundant peptide (16.96 mg/g DW) in prolamins. The results indicated that prolamin hydrolysates from DDSG could be served as a source of ACE inhibitory peptides. View Full-Text
Keywords: distilled spent grain (DSG); baijiu (Chinese liquor); ACE inhibitory peptides; Prolamin isolate hydrolysates; RP-HPLC; AVQ; YPQ distilled spent grain (DSG); baijiu (Chinese liquor); ACE inhibitory peptides; Prolamin isolate hydrolysates; RP-HPLC; AVQ; YPQ
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MDPI and ACS Style

Wei, D.; Fan, W.; Xu, Y. In Vitro Production and Identification of Angiotensin Converting Enzyme (ACE) Inhibitory Peptides Derived from Distilled Spent Grain Prolamin Isolate. Foods 2019, 8, 390.

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