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Assessment of RNAlater® as a Potential Method to Preserve Bovine Muscle Proteins Compared with Dry Ice in a Proteomic Study

1
Teagasc Food Research Centre, Ashtown, Dublin D15KN3K, Ireland
2
School of Food and Nutritional Sciences, University College Cork, Cork T12 K8AF, Ireland
3
Department of Chemistry, Khalifa University, Abu Dhabi PO Box 127788, UAE
*
Author to whom correspondence should be addressed.
Foods 2019, 8(2), 60; https://doi.org/10.3390/foods8020060
Received: 9 January 2019 / Revised: 31 January 2019 / Accepted: 2 February 2019 / Published: 5 February 2019
RNAlater® is regarded as a potential preservation method for proteins, while its effect on bovine muscle proteins has rarely been evaluated. Bovine muscle protein samples (n = 12) collected from three tender (Warner–Bratzler shear force: 30.02–31.74 N) and three tough (Warner–Bratzler shear force: 54.12–66.25 N) Longissimus thoracis et lumborum (LTL) samples, preserved using two different sampling preservation methods (RNAlater® and dry ice), at two post mortem time points (day 0 and day 14), were characterized using one-dimensional electrophoresis. Fourteen bands with molecular weights ranging from 15 to 250 kDa were verified, both in the dry ice and RNAlater® storage groups, at each time point, using image analysis. A shift from high to low molecular weight fragments, between day 0 and day 14, indicated proteolysis of the muscle proteins during post mortem storage. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses and database searching resulted in the identification of 10 proteins in four bands. Protein profiles of muscle preserved in RNAlater® were similar to those of muscle frozen on dry ice storage, both at day 0 and day 14. The results demonstrate that RNAlater® could be a simple and efficient way to preserve bovine muscle proteins for bovine muscle proteomic studies. View Full-Text
Keywords: muscle proteins; one-dimensional electrophoresis; bovine proteomics; LC-MS/MS; sample preparation muscle proteins; one-dimensional electrophoresis; bovine proteomics; LC-MS/MS; sample preparation
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Zhu, Y.; Mullen, A.M.; Rai, D.K.; Kelly, A.L.; Sheehan, D.; Cafferky, J.; Hamill, R.M. Assessment of RNAlater® as a Potential Method to Preserve Bovine Muscle Proteins Compared with Dry Ice in a Proteomic Study. Foods 2019, 8, 60.

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