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Open AccessArticle

Seasonal Variations in the Chemical Composition of Liangshan Olive Leaves and Their Antioxidant and Anticancer Activities

1
College of Life Sciences, Sichuan Agricultural University, Yaan 625014, China
2
College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China
3
College of Agricultural Science, Xichang University, Xichang 615000, China
*
Author to whom correspondence should be addressed.
Foods 2019, 8(12), 657; https://doi.org/10.3390/foods8120657
Received: 24 October 2019 / Revised: 26 November 2019 / Accepted: 6 December 2019 / Published: 8 December 2019
The seasonal changes in the chemical composition of Olea europaea leaves from January to December at Liangshan (China) have been investigated. The highest total phenolic content (TPC), total flavonoid content (TFC), and free amino acid content (FAAC) levels were found in May and December, while the lowest levels were detected in April and September. The soluble protein content (SPC) and the soluble sugar content (SSC) were highest in spring but lowest in summer and winter. The levels of major phenolic compounds, including oleuropein, and luteolin-4’-O-glucoside, followed by apigenin-7-O-glucoside, quercetin, rutin, luteolin, and apigenin, increased during spring and winter but decreased during summer and autumn. In addition, phenolic extracts (PEs) showed dose-dependent antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and superoxide radical scavenging activity assays; the reducing power was tested. The anticancer activities of PE at various concentrations were assessed by a cell counting kit-8 (CCK-8), and the IC50 (50% effective concentration) to HEK293, HeLa, and S180 cells were 841.48, 7139, and 457.69 μg/mL, respectively. PE-treated S180 cells inhibited proliferation through activation of caspase-3/9 and disruption of the mitochondrial membrane potential. Thus, PE in Liangshan olive leaves possessed strong antioxidant and anticancer potential, and spring and winter were determined as optimal harvesting seasons. View Full-Text
Keywords: Liangshan olive leaves; chemical composition; phenolic extracts; anticancer activity Liangshan olive leaves; chemical composition; phenolic extracts; anticancer activity
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Wang, B.; Qu, J.; Feng, S.; Chen, T.; Yuan, M.; Huang, Y.; Liao, J.; Yang, R.; Ding, C. Seasonal Variations in the Chemical Composition of Liangshan Olive Leaves and Their Antioxidant and Anticancer Activities. Foods 2019, 8, 657.

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