Protein Determination—Method Matters
AbstractThe reported protein content of foods depends on the analytical method used for determination, making a direct comparison between studies difficult. The aim of this study was to examine and compare protein analytical methods. Some of these methods require extraction preceding analysis. The efficacy of protein extraction differs depending on food matrices and thus extraction yield was determined. Overall, most analytical methods overestimated the protein contents. The inaccuracies were linked to indirect measurements, i.e. nitrogen determination and subsequent conversion to protein, or interference from other chemical substances. Amino acid analysis is the only protein analysis method where interfering substances do not affect the results. Although there is potential for improvement in regards to the hydrolysis method, we recommend that this method should be the preferred for food protein determination. View Full-Text
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Mæhre, H.K.; Dalheim, L.; Edvinsen, G.K.; Elvevoll, E.O.; Jensen, I.-J. Protein Determination—Method Matters. Foods 2018, 7, 5.
Mæhre HK, Dalheim L, Edvinsen GK, Elvevoll EO, Jensen I-J. Protein Determination—Method Matters. Foods. 2018; 7(1):5.Chicago/Turabian Style
Mæhre, Hanne K.; Dalheim, Lars; Edvinsen, Guro K.; Elvevoll, Edel O.; Jensen, Ida-Johanne. 2018. "Protein Determination—Method Matters." Foods 7, no. 1: 5.
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