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Article

The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice

1
Jinhua Key Laboratory of Biotechnology on Specialty Economic Plants, College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
2
China-Mozambique “Belt and Road” Joint Laboratory on Smart Agriculture, Zhejiang Normal University, Jinhua 321004, China
3
Yunnan Key Laboratory of Genetic Improvement of Herbal Oil Crops, Industrial Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650225, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Foods 2025, 14(19), 3396; https://doi.org/10.3390/foods14193396
Submission received: 7 August 2025 / Revised: 8 September 2025 / Accepted: 29 September 2025 / Published: 30 September 2025
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)

Abstract

Polygonatum cyrtonema Hua, a plant with a long history of consumption in China, serves both medicinal and edible purposes, and it exhibits numerous pharmacological effects, including promoting kidney health and enhancing immune function. However, the effect and molecular mechanism of Polygonatum cyrtonema polysaccharides (PCPs) on hyperuricemia have not yet been reported. The hyperuricemic mice model was induced by the intraperitoneal injection of potassium oxonate (PO, 300 mg/kg), combined with the intragastric administration of hypoxanthine (HX, 300 mg/kg). Biochemical assays in mice revealed that PCPs markedly lowered high serum uric acid levels, suppressed xanthine oxidase (XOD) activity, and reduced the expression of inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Western blot analysis demonstrated that PCPs downregulated urate transporter 1 (URAT1), while H&E staining showed that PCPs effectively restored renal histological integrity. Here, we isolated and identified the PCPs, which consist mainly of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose, with a molar mass ratio of 0.5:2.15:0.47:16.58:3.66:1.09. Furthermore, the galactose residue that docked with both XOD and URAT1 molecules forms more hydrogen bonds and exhibits a lower binding energy, which enables the improved regulation of both targets. We have demonstrated for the first time the improving effect of PCPs on hyperuricemia, and revealed their regulatory mechanisms by modulating xanthine oxidase, inflammatory factors, and uric acid transporters. This study not only provides new insights into the anti-hyperuricemic activity of PCPs in mice, but also lays a foundation for its potential application in the functional foods of anti-hyperuricemia.
Keywords: Polygonatum cyrtonema; polysaccharides; hyperuricemia; molecular mechanism; xanthine oxidase; URAT1; inflammatory factors Polygonatum cyrtonema; polysaccharides; hyperuricemia; molecular mechanism; xanthine oxidase; URAT1; inflammatory factors

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MDPI and ACS Style

Pu, S.; Gong, J.; Sun, M.; Hu, Z.; Wu, Z. The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice. Foods 2025, 14, 3396. https://doi.org/10.3390/foods14193396

AMA Style

Pu S, Gong J, Sun M, Hu Z, Wu Z. The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice. Foods. 2025; 14(19):3396. https://doi.org/10.3390/foods14193396

Chicago/Turabian Style

Pu, Shoucheng, Jufang Gong, Meihao Sun, Zunhong Hu, and Zhihua Wu. 2025. "The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice" Foods 14, no. 19: 3396. https://doi.org/10.3390/foods14193396

APA Style

Pu, S., Gong, J., Sun, M., Hu, Z., & Wu, Z. (2025). The Molecular Mechanism of Polysaccharides from Polygonatum cyrtonema Hua in Improving Hyperuricemia by Regulating Key Targets of Uric Acid Metabolism in Mice. Foods, 14(19), 3396. https://doi.org/10.3390/foods14193396

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