Characterization of Genomic, Physiological, and Probiotic Features of Lactiplantibacillus plantarum JS21 Strain Isolated from Traditional Fermented Jiangshui
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation of Bacterial Strain and Growth Conditions
2.2. DNA Extraction, Identification, Whole Genome Sequencing, and Assembly
2.3. Bioinformatic Analysis
2.4. Carbohydrate Fermentation
2.5. Determination of Antibiotic Susceptibility
2.6. Determination of Probiotic Properties
3. Results and Discussion
3.1. Functional Genomic Characterization
3.2. Prophages, Related Horizontal Gene Transfer, and Mobilome
3.3. Phenotypic Antibiotic Resistance and Safety-Related Gene Assessment
3.4. Carbohydrate Fermentation Patterns and Active Enzymes
3.5. Secondary Metabolite Biosynthetic Gene Clusters
3.6. Probiotic Properties
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Type | Name | Size (bp) | G+C (%) | Genes | Pseudogenes (Total) | Protein | rRNA | tRNA | Non-Coding RNA | CRISPR_Array | CRISPR_Repeat | CRISPR_Spacer |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Chromosome | - | 3.126.700 | 44.79 | 3090 | 223 | 2747 | 64 | 16 | 40 | 6 | 85 | 79 |
Plasmid | pWS01 | 72.513 | 42.33 | 79 | 12 | 67 | - | - | - | - | - | - |
Plasmid | pWS02 | 71.633 | 40.97 | 71 | 6 | 65 | - | - | - | - | - | - |
Plasmid | pWS03 | 54.200 | 40.14 | 55 | 10 | 45 | - | - | - | - | - | - |
Plasmid | pWS04 | 51.130 | 41.74 | 70 | 5 | 65 | - | - | - | - | - | - |
Plasmid | pWS05 | 47.792 | 43.9 | 42 | 8 | 34 | - | - | - | - | - | - |
Sugars | JS21 |
---|---|
Control | − |
Glycerol | + |
Erythritol | − |
D-Arabinose | − |
L-Arabinose | − |
D-Ribose | + |
D-Xylose | − |
L-Xylose | − |
Adonitol | − |
Methyl-β-D-xylopyranoside | − |
D-Galactose | + |
D-Glucose | + |
D-Fructose | + |
D-Mannose | + |
D-Sorbose | − |
D-Rhamnose | − |
Dulcitol | − |
Inositol | + |
D-Mannitol | + |
D-Sorbitol | + |
Methyl-α-D-mannopyranoside | + |
Methyl-α-D-glucopyranoside | − |
N-Acetylglucosamine | + |
Amygdalin | + |
Arbutin | + |
Esculin ferric citrate | + |
Salicin | + |
D-Cellobiose | + |
D-Maltose | + |
D-Lactose | + |
D-Melibiose | + |
D-Sucrose | + |
D-Trehalose | + |
Inulin | − |
D-Melezitose | + |
D-Raffinose | − |
Amidon (Starch) | − |
Glycogen | − |
Xylitol | − |
Gentiobiose | + |
D-Turanose | − |
D-Lyxose | − |
D-Tagatose | − |
D-Fucose | − |
L-Fucose | − |
D-Arabitol | − |
L-Arabitol | − |
Gluconate | − |
2-Keto-gluconate | − |
5-Keto-gluconate | − |
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Liu, Y.; Wang, S.; Wang, L.; Lu, H.; Zhang, T.; Zeng, W. Characterization of Genomic, Physiological, and Probiotic Features of Lactiplantibacillus plantarum JS21 Strain Isolated from Traditional Fermented Jiangshui. Foods 2024, 13, 1082. https://doi.org/10.3390/foods13071082
Liu Y, Wang S, Wang L, Lu H, Zhang T, Zeng W. Characterization of Genomic, Physiological, and Probiotic Features of Lactiplantibacillus plantarum JS21 Strain Isolated from Traditional Fermented Jiangshui. Foods. 2024; 13(7):1082. https://doi.org/10.3390/foods13071082
Chicago/Turabian StyleLiu, Yang, Shanshan Wang, Ling Wang, Hongzhao Lu, Tao Zhang, and Wenxian Zeng. 2024. "Characterization of Genomic, Physiological, and Probiotic Features of Lactiplantibacillus plantarum JS21 Strain Isolated from Traditional Fermented Jiangshui" Foods 13, no. 7: 1082. https://doi.org/10.3390/foods13071082