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Article

Flavor Variations in Precious Tricholoma matsutake under Different Drying Processes as Detected with HS-SPME-GC-MS

1
Germplasm Bank of Wild Species & Yunnan Key Laboratory for Fungal Diversity and Green Development, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
2
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Ministry of Education, Kunming 650224, China
3
College of Resources and Environment, Southwest University, Chongqing 400715, China
*
Author to whom correspondence should be addressed.
Foods 2024, 13(13), 2123; https://doi.org/10.3390/foods13132123
Submission received: 27 May 2024 / Revised: 23 June 2024 / Accepted: 1 July 2024 / Published: 3 July 2024
(This article belongs to the Section Food Analytical Methods)

Abstract

:
By employing headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS), this study displayed the compositional changes in volatile organic compounds (VOCs) in Tricholoma matsutake samples subjected to hot-air drying (HAD) and vacuum freeze-drying (VFD) processes from their fresh samples. A total of 99 VOCs were detected, including 2 acids, 10 aldehydes, 10 alcohols, 13 esters, 12 ketones, 24 alkanes, 14 olefins, 7 aromatic hydrocarbons, and 7 heterocyclic compounds. Notably, the drying process led to a decrease in most alcohols and aldehydes, but an increase in esters, ketones, acids, alkanes, olefins, aromatic, and heterocyclic compounds. Venn diagram (Venn), principal component analysis (PCA), and partial least squares-discriminant analysis (PLS-DA) analyses enabled an easy and rapid distinction between the VOC profiles of T. matsutake subjected to different drying methods. Among the identified VOCs, 30 were designated as marker VOCs indicative of the employed drying process. And the VFD method was more capable of preserving the VOCs of fresh T. matsutake samples than the HAD method. Benzaldehyde, 1-Octen-3-ol, 3-Octanol, and (E)-2-Octen-1-ol were identified as markers for FRESH T. matsutake. Conversely, (E)-3-Hexene, lavender lactone, and α-Pinene were associated with VFD T. matsutake. For HAD T. matsutake, olefins, pyrazine, and esters, particularly ocimene, 2,5-Dimethyl-pyrazine, and methyl cinnamate, significantly contributed to its particularities. The results from this present study can provide a practical guidance for the quality and flavor control of volatile organic compounds in preciously fungal fruiting bodies by using drying processes.

1. Introduction

Tricholoma matsutake, a member of ectomycorrhizal fungus, is a highly valued delicacy for its distinctive flavor and taste. The volatile organic compounds (VOCs) of T. matsutake mainly include alcohols, aldehydes, acids, esters, ketones, sulfur compounds, olefins, and C8 aliphatic compounds [1]. The differences of T. matsutake VOCs have been investigated under different fried heating/pan-frying temperatures and times [2,3], geographical origins [4,5], grades [6], and cold storage times [7]. Furthermore, the differences of volatile compounds in pileus and stipe [4,8], fresh- and hot-air drying (HAD) [9], and raw and convection-broiler-cooked [10] T. matsutake were also observed. C8 compounds (1-Octen-3-ol, 1-Octanol, (E)-2-octen-1-ol, 1-Octen-3-one, 3-Octanol, 3-Octanone, and benzeneacetaldehyde) and methyl cinnamate are the most frequent VOCs of T. matsutake. In particular, methyl cinnamate provides the characteristic aroma components of T. matsutake samples.
An important factor in determining the quality and flavor of dried T. matsutake is closely related to the drying method [9]. Fresh T. matsutake has a high water content and is not resistant to storage, making it prone to various physiological changes after being picked. If no protection and anti-corrosion measures are carried out, T. matsutake is easily deteriorated, leading to the loss of nutritional components and a decrease in its edible and market value. Therefore, drying is usually an important storage method for T. matsutake to effectively control moisture and extend its food shelf life [7]. During the drying and dehydration process, the concentration of C8 compounds is significantly reduced due to the Maillard reaction or the destruction of tissue cells [11]. This process also leads to the formation of some volatile compounds, including hexaldehyde, heptal, 2(5H)-furanone, acetophenone, nonylaldehyde, phenacetaldehyde, etc., resulting in a partial loss of flavor and reduced quality of T. matsutake; thus, the selection of different drying methods is of great significance for preserving the flavor of T. matsutake.
Due to the limited research on the impact of various drying processes on volatile organic compounds of T. matsutake, in this present study, fresh T. matsutake was used as the raw material, and dried T. matsutake was prepared with hot-air drying (HAD) and vacuum freeze-drying (VFD) processes. Headspace solid-phase microextraction (HS-SPME) technology, known for its high selectivity, enrichment efficiency, and rapid analysis capabilities, was employed for extraction and analysis to assess the impact of various drying methods on T. matsutake flavor. The aim of this study is to provide a theoretical reference for the further process and utilization of T. matsutake and a practical basis for promoting its commercial application.

2. Materials and Methods

2.1. Fungal Materials and Sample Production

T. matsutake samples were obtained from the Diqing Tibetan Autonomous Prefecture, Yunnan, Southwest China. FRESH samples were stored at room temperature; VFD (vacuum freeze drying, FD-1A-50, BIOCOOL, Beijing, China) samples were frozen at −80 °C for 24 h, and then vacuum freeze-dried for 24 h at −40 to −50 °C, vacuum 10 Pa; HAD (hot air drying, GZX-GF 101-3 BS, Yuejin Medical Co., Shanghai, China) samples were weighed at a certain quantity, cut into uniform slices, dried for 4 h at 60 to 70 °C, and then dried for 2 h at 70 to 100 °C (Figure 1).

2.2. Headspace Solid-Phase Microextraction (HS-SPME)

Samples were extracted using a manual headspace sampling system, equipped with a 50/30 µm DVB/CAR/PDM fiber (Supelco, Bellefonte, PA, USA). Fresh samples were chopped and weighed at 5.0 g. The samples were placed in 40 mL headspace vials, pre-equilibrated at 45 °C for 5 min, and then extracted for 40 min at the same temperature. After extraction, the fiber was immediately inserted into the injection port of GC-MS for thermal desorption at 250 °C for 10 min.

2.3. Gas Chromatography–Mass Spectrometry (GC-MS) Analysis

Gas chromatography–mass spectrometry (7890A-5975C, Agilent, Santa Clara, CA, USA) with a capillary column DB-5MS (30 m × 0.25 mm, 0.25 μm, Agilent, USA) was utilized. The injection port was operated in splitless mode at 250 °C. The following chromatographic separations were performed: 40 °C held for 5 min, increased to 180 °C at a rate of 5 °C/min and held for 2 min, and then to 260 °C at a rate of 10 °C/min. Helium was used as the carrier gas with a flow rate of 1.0 mL/min. The operating conditions for the MS system were as follows: the ion source was set at 230 °C, and the electron ionization mode was at 70 eV with mass ranges from 35 to 500 m/z.

2.4. Statistical Analysis

Each component underwent NIST11 library search, and data were analyzed using MSD ChemStation software (Agilent Technologies, version G1701EA E. 02. 02. 1431). For each analyte, its relative mass fraction was calculated by the peak area normalization method.
Data were reported as means ± standard deviation (SD). There were three replicates for each treatment, and p-values for differences between different treatments within the same species were examined using Student’s t-test (p ≤ 0.05). Principal component analysis (PCA) and partial least squares–discriminant analysis (PLS-DA) were performed using Simca-p 14.1 software, while Origin 2021 was utilized for correlation analysis and visualization of any differences between samples.

3. Results

3.1. Changes in HS-SPME-GC-MS of T. matsutake with Differing Drying Processes

The varying VOCs in T. matsutake with differing drying processes were analyzed using HS-SPME-GC-MS. The signals in the 20–25 min retention time were rapidly increased in VFD and HAD while remaining consistent among the ion chromatograms of the other samples (Figure 2). A mass of VOCs was indicated to be released during the drying processes. Herein, a total of 99 different volatile compounds were identified by the HS-SPME-GC-MS analysis, including 2 acids, 10 aldehydes, 10 alcohols, 13 esters, 12 ketones, 24 alkanes, 14 olefins, 7 aromatic hydrocarbons, and 7 heterocyclic compounds (Table 1). The relative amount of each compound was obtained by its peak area normalization.

3.2. Variation in VOCs of T. matsutake

3.2.1. Changes in the Types and Relative Contents of VOCs

A total of 66 VOCs were detected in the VFD group, with a high relative content of 36.82% hydrocarbons (alkanes and olefins), 32.38% alcohols, and 21.01% esters, respectively. In the HAD group, a total of 65 volatile components were detected, with 54.60% esters, 23.67% hydrocarbons, and 10.89% ketones, respectively. In the FRESH group, a total of 44 VOCs were detected, with 89.47% alcohols, but without acid substances (Table 1 and Figure 3). Different drying methods had a significant effect on the VOCs in T. matsutake. After drying, the relative contents of volatile components in T. matsutake increased, while both alcohols and aldehydes were decreased. However, the relative content of other components, such as esters, increased. Esters were formed by the interaction of alcohols and free fatty acids resulting from fat oxidation [12].

3.2.2. Analysis of Major VOCs in Different-Drying T. matsutake

The main VOCs of the FRESH group were 1-Octen-3-ol (68.67%), 3-Octanol (16.38%), (E)-2-Octen-1-ol (3.33%), methyl cinnamate (2.07%), and benzaldehyde (1.16%). The main volatile components of the VFD group were 1-Octen-3-ol (31.41%), methyl cinnamate (19.93%), β-Barbatene (15.02%), (E)-3-Hexene (6.65%), dodecane (4.14%), undecane (3.75%), 1-Octen-3-one (1.95%), octane (1.45%), and 5-Ethenyldihydro-5-methyl-2(3H)-furanone (1.08%). The main VOCs of the HAD group were methyl cinnamate (52.76%), β-Barbatene (7.64%), 2(5H)-Furanone (6.38%), ocimene (5.34%), dodecane (3.80%), 2,5-Dimethylpyrazine (2.63%), benzaldehyde (1.85%), and octane (1.07%). Methyl cinnamate was the most important volatile component of T. matsutake. The relative content of methyl cinnamate was the highest at 52.76% in the HAD group, followed by 19.93% in the VFD group and 2.07% in the FRESH group. 1-Octen-3-ol was the major common VOC in the FRESH and VFD groups, comprising 68.67% and 31.41%, respectively (Table 1).

3.2.3. Analysis of the Unique and Common VOCs in Different Drying Processes of T. matsutake

From Table 2 and Figure 4, the VFD, HAD, and FRESH groups of T. matsutake contained 17, 18, and 7 unique components, with respective relative contents of 7.2%, 4.49%, and 0.58%. The VFD group was divided into seven alkanes, four aromatics, two heterocyclics, and one of each acid, aldehyde, ester and olefin. The HAD group was divided into five alkanes, three heterocytypes, two kinds of alcohol, ketone and olefin, and one kind of acid, aldehyde, ester and aromatic. And the FRESH group was divided into three aldehydes and one of alcohol, ester, ketone and alkanes. These three groups had 19 common components, with relative contents of 73.53% (VFD), 69.19% (HAD), and 80.95% (FRESH).

3.3. Characteristic VOCs via PCA and PLS-DA

To further understand the differences in the VOCs under three key T. matsutake processing points, a total of 99 significantly different volatiles among samples were used to run the PCA (Figure 5A). PC1 and PC2 were the qualitative and quantitative analysis of VOCs in the spectrum, with contribution rates of 47.1% and 29.9%, respectively. There was no overlap among the three sample groups in Figure 5A, thereby indicating significant differences in the VOCs among the sample groups. The samples with different drying methods can thus be well distinguished by PCA. The value of PC2 was increased in the following order: HAD < VFD < FRESH. A large separation between HAD and the other drying groups implied significant changes in the VOCs caused by the HAD method. Conversely, the smaller separation between FRESH and VFD indicated that the effects of drying treatments on their chemical profiles were similar. Therefore, the results revealed that HS-SPME-GC-MS coupled with PCA can rapidly distinguish T. matsutake via different drying treatments, and thus be a promising quality control method of the three processes. In Figure 5B, the differentiation among the T. matsutake samples from three drying methods was more clearly demonstrated through score plots combined with loading plots, effectively illustrating the correlations between the 99 VOCs and the samples.
As show in Figure 5B, the VFD samples were predominantly located in the I quadrant, with the VFD samples being characterized by (E)-2-Octenal (5), atropaldehyde (6), decanal (8), (E,E)-2,4-Decadienal (11), 3-Octanol (15), γ-Valerolactone (24), 2(5H)-Furanone (36), 2-Cyclopropyl-butane (48), 3-Methyl-nonane (50), 4,7-Dimethyl-undecane (52), methyl-cyclooctane (58), and naphthalene (89). The FRESH samples were scattered in quadrant II, and positively corrected with benzeneacetaldehyde (4), (E,E)-2,4-nonadienal (9), diisobutyl phthalate (34), 2,2,5-Trimethyl-3-hexanone (40), acetophenone (43), 2-Methyl-pentane (51), undecane (54), pentyl-cyclohexane (55), and 3-Methyl-dodecane (64). The HAD samples (in the III quadrant) were more correlated to butanoic acid (2), nerolidol (22), tetrahydrofurfuryl propionate (25), caprylic acid methyl ester (27), 2-Ethylhexyl hexyl sulfite (29), 1-Octen-3-one (37), 1-Hepten-3-one (38), 3-Octen-2-one (42), 1-Ethyl-1-methyl-cyclopentane (53), tetradecane (67), 1,3-Octadiene (72), 2,4-Dimethyl-1-heptene (73), α-Pinene (76), 2,4-Di-tert-butylphenol (92), and 1,1-Dioxide-2-methylthiolane (97).
Upon identifying a classification trend among the three matsutake samples via PCA, a further examination into the variables driving these differences was conducted through discriminant analysis using PLS-DA. The VIP score quantified the influence of different compounds in the PLS-DA model, with higher VIP values signifying a greater contribution to the classification of T. matsutake across the three treatments. The VIP score map from the PLS-DA model (Figure 5C) identified 57 volatile substances as key differential components capable of distinguishing the three processing methods of T. matsutake (VIP > 1, p < 0.05), including butanoic acid (2), nerolidol (22), tetrahydrofurfuryl propionate (25), caprylic acid methyl ester (27), methyl oct-2-enoate (28), diisobutyl phthalate (34), 1-Octen-3-one (37), 3-Octanone (39), 2,2,5-Trimethyl-3-hexanone (40), 3-Octen-2-one (42), pentyl-cyclohexane (55), 2,6,10-Trimethyl-dodecane (66), (+)-Sativen (83), 1,1-Dioxide-2-methylthiolane (97), etc. This analysis, along with Table 1, highlights significant variations in the relative contents of these VOCs across the matsutake samples, underlining their importance in defining the unique aroma profiles of each sample.

4. Discussion

Differences were found in the types and contents of VOCs of T. matsutake compared with a previous study. These variances could be attributed to differences in the fried heating/pan-frying temperatures and times [2,3], cold storage times [7], and geographical origins [4,5,13], etc.
The VOCs mostly originate from the chemical or enzymatic oxidation of unsaturated fatty acids, followed by interactions with proteins, peptides, and free amino acids. Other volatile compounds result from the Strecker degradation of free amino acids and Maillard reactions [14].
The contents of C8 compounds, especially 1-octen-3-ol, 3-octanone, 1-octanol, 3-octanol, and (E)-2-octen-1-ol, were significantly decreased after the drying process. Yang et al. [15] reported the degradation of C8 components during heat treatment. Due to the destruction of the cell wall and cytoplasm, more various intracellular components are released from cells and participate in the reaction during drying while some VOCs might be formed.
Aldehydes significantly contribute to the formation of flavors in edible fungi, characterized by their abundant presence and relatively low odor thresholds [16]. Our analysis revealed that aldehydes varied in quantity across the samples, with five compounds identified in both VFD and HAD samples, and eight compounds in the FRESH samples. Notably, aldehydes constituted the highest proportion of volatile compounds in the FRESH sample, accounting for up to 4.90% of the relative content. Similarly, substantial quantities of aldehydes were detected in the other two mushroom samples. All identified aldehyde compounds were classified as unsaturated aldehydes, commonly recognized as the oxidation products of unsaturated fatty acids [17]. The variation in aldehyde content and composition across samples was primarily attributed to the different drying methods employed. In the context of actual production, processing conditions, particularly drying methods, significantly influence the concentration of aldehydes [18]. Contrary to expectations, the quantity of aldehydes in our study exhibited a decrease, highlighting the impact of processing techniques on aldehyde profiles.
Alcohols are primarily formed through the lipid oxidation of polyunsaturated fatty acids, which typically have a higher threshold and contribute to a soft and sweet aroma [13]. In the VFD, HAD, and FRESH samples, 7, 8, and 5 alcohols were observed, respectively. Simultaneously, alcohols were the most abundant group in the FRESH sample, occupying 89.47% of the total peak area. In addition to fatty alcohols, terpenes such as linalool and nerolidol were also included, which were detected in the samples. Among these samples, the sample from the FRESH group contained the highest alcohol content (89.47% at room temperature), which decreased with increasing drying temperatures: VFD (32.38% at −40 °C to −50 °C for 24 h) and HAD (4.13% at 60 °C to 70 °C for 4 h; 70 °C to 100 °C for 2 h). These results were in accordance with other reports that focused on the change in alcohols in edible fungi at different heating temperatures. Additionally, (E)-2-octen-1-ol, 1-octene-3-ol, and 1-octanol were detected in all the three T. matsutake samples. The alcohol content was decreased after the drying processes. It could contribute to the development of matsutake mushroom flavor. The 1-octene-3-ol alcohol group was identified as the major volatile compound found in raw mature T. matsutake. This aliphatic unsaturated alcohol is beneficial for enhancing the manifestation of mushroom flavor. 1-Octen-3-ol, with a typical odor reminiscent of mushrooms, lavender, rose and hay, has been detected and reported in most edible fungi [6,19]. 1-octen-3-ol is the product of autoxidation and/or the enzymatic oxidation and cleavage of linoleic acid in mushroom [20]. Alcohols are a class of VOCs that have been detected in raw mushroom and mushroom products like Volvariella volvacea, shiitake mushrooms, and Agaricus bisporus [21].
Esters mainly exist in fruits and exhibit a sweet and fruity flavor, which is associated with the oxidation of unsaturated fatty acids [22]. The content of esters varied greatly in T. matsutake samples at different drying temperatures. The total content of esters was increased with the increased in drying temperature, ranging from 3.85% to 54.60% in samples dried using FRESH, VFD, and HAD methods. Processing methods, such as drying, could lead to an increase in esters [18]. Among these esters, the contents of tetrahydrofurfuryl propionate and methyl cinnamate in dried samples (VFD and HAD) was higher than these in the FRESH sample. Tetrahydrofurfuryl propionate imparts a fruit aroma, while methyl cinnamate impacts a strawberry aroma.
A lower content of ketones was found in T. matsutake, accounting for 0.40–10.89% of the total VOCs. The content of ketones showed a relative increasing trend with the increase in drying temperature. Several ketones were generated during the drying process as a result of the thermal degradation of amino acids or the thermal oxidation of polyunsaturated fatty acids, such as leucine, phenylalanine, and threonine [23,24]. Different amounts of ketones were detected, with 7, 11, and 5 compounds identified in the VFD, HAD, and FRESH samples, respectively. Ketones were observed to be the most abundant compounds in HAD samples, accounting for 10.89% of the peak area, while in other samples, they were relatively low. Ketones are products of the decomposition of esters or the oxidation of alcohols [25].
Hydrocarbons (alkanes and olefins) are generally not considered to have an aroma contribution due to their relatively high odor threshold. However, alkanes could help enhance the flavor of food [15]. The hydrocarbons content was increased at both low and high drying temperatures. The VFD samples (low temperature) showed a more significant increase compared to the HAD samples (high temperature), with contents of 36.82% and 23.67% of hydrocarbons, respectively. This phenomenon can be attributed to the dynamic equilibrium between the cracking reaction of alkoxyl radicals and the loss of volatile components at higher temperatures [25]. Some alkanes and olefins were also detected in the samples, accounting for 15, 26, and 25 compounds in the FRESH, VFD, and HAD samples, respectively.
All samples also contained a very small number of aromatic hydrocarbons, which were identified as six compounds in VFD, two compounds in HAD, and one compound in FRESH samples. A minimal number of acid compounds was only detected in VFD (1 compound, 0.23%) and HAD (1 compound, 0.18%) samples, but not detectable in the FRESH samples. These acid compounds and aromatic hydrocarbons have not been reported in T. matsutake by other references [8]. Eleven acids were identified from different geographical origins of T. matsutake [24], which differed from our results.
Heterocyclic compounds, especially pyrazine, are an important source of the unique VOCs of T. matsutake, which has a strong odor intensity with nutty and roasted flavors [10]. It was observed that 2,5-Dimethyl pyrazine changed with the increase in drying temperature for the VFD (0.11%) and HAD (2.63%) samples.
By analyzing the relative contents of common VOCs in different drying methods (FRESH (80.95%) > VFD (73.53%) > HAD (69.19%), we can see that the VFD samples were relatively close to the FRESH samples. As seen from the Venn diagram of the VOCs, the VFD samples had a greater amount of common VOCs with the FRESH samples compared to the HAD samples. (E)-2-octenal, 1-Octen-3-ol, 1-Octen-3-one, 3-Octanol and 3-Octen-2-one had a characteristic mushroom-like odor and were recognized as a key odorant in forming the distinctive mushroom aroma, playing a role in the reconciliation and complementation of the flavor of T. matsutake samples [24]. And they were much better retained in the VFD than the HAD sample. Especially, the relative amount of 1-Octen-3-ol and 1-Octen-3-one in VFD samples (31.41%, 1.95%) were much more abundant than HAD samples (0.77%, 0.72%). Moreover, the smaller separation between the FRESH and VFD samples indicated that the effects of drying treatments on their chemical profiles were similar. Thus, the VFD method was more capable of preserving the VOCs of fresh T. matsutake samples than the HAD method.

5. Conclusions

This study focused on the diversity of the VOCs in T. matsutake samples under fresh, hot-air-drying, and vacuum freeze-drying treatments. SPME-GC-MS was successfully employed to identify flavor compounds formed after different drying treatments of T. matsutake. A total of 99 flavor substances were identified across these three treatments of T. matsutake, including acids, aldehydes, alcohols, ketones, esters, alkanes, olefins, aromatic hydrocarbons and heterocycle compounds. In addition, the PCA analysis from GC-MS showed that samples from different drying processes could be distinguished by their VOCs, and the VFD method was more capable of preserving the VOCs of fresh T. matsutake samples than the HAD method. Based on the VIP score diagram of the PLS-DA model on VOCs in these samples, 30 VOCs were identified as potentially contributing to the aroma of T. matsutake. Benzaldehyde, 1-Octen-3-ol, 3-Octanol, and (E)-2-Octen-1-ol were identified as the primary compounds responsible for the mushroom aromas of fresh T. matsutake before the drying process. This study demonstrated the potential of HS-SPME-GC-IMS in combination with PCA and PLS-DA as a reliable analytical screening technique to quickly and sensitively identify and classify the VOCs of T. matsutake. Results from this present study can provide a theoretical and practical basis for the quality control of flavor in the processing of preciously edible fungal products.

Author Contributions

Conceptualization, F.Z., B.L. and F.Y.; methodology, B.L. and F.Z.; software, F.Z.; validation, F.Z. and F.Y.; investigation, F.Z.; supervision, F.Y.; data curation, F.Z. and B.L.; writing—original draft, F.Z.; writing—review and editing, F.Y. and X.H.; funding acquisition, F.Z. and F.Y. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Yunnan Key Project of Science and Technology (202202AE090001), Postdoctoral Directional Training Foundation of Yunnan Province (E23174K2), and Postdoctoral Research Funding Projects of Yunnan Province (E2313442), China.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The original contributions presented in the study are included in the article; further inquiries can be directed to the corresponding author and the first author.

Conflicts of Interest

The authors declare no conflicts of interest.

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Figure 1. The fruiting bodies of Tricholoma matsutake.
Figure 1. The fruiting bodies of Tricholoma matsutake.
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Figure 2. Total ion chromatograms of T. matsutake during the drying process. (Number of peaks is shown in Table 1).
Figure 2. Total ion chromatograms of T. matsutake during the drying process. (Number of peaks is shown in Table 1).
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Figure 3. Classification analysis of VOCs in dry T. matsutake after different drying processes.
Figure 3. Classification analysis of VOCs in dry T. matsutake after different drying processes.
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Figure 4. The Venn diagram of VOCs.
Figure 4. The Venn diagram of VOCs.
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Figure 5. PCA and PLS-DA analysis of VOCs of dry T. matsutake after in different drying methods. (A) PCA analysis of VOCs of T. matsutake after different drying treatments. (B) PCA loading diagram. (Numbers in the figure are the same as those in Table 1). (C) VIP score diagram of PLS-DA model. (Numbers in the figure are the same as those in Table 1).
Figure 5. PCA and PLS-DA analysis of VOCs of dry T. matsutake after in different drying methods. (A) PCA analysis of VOCs of T. matsutake after different drying treatments. (B) PCA loading diagram. (Numbers in the figure are the same as those in Table 1). (C) VIP score diagram of PLS-DA model. (Numbers in the figure are the same as those in Table 1).
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Table 1. The relative amounts of volatile compounds in different-drying T. matsutake.
Table 1. The relative amounts of volatile compounds in different-drying T. matsutake.
No.CASCompoundFormulaMWRT [min]Relative Amount %
VFDHADFRESH
Acids
1503-74-23-Methyl-butanoic acidC5H10O2102.15.520.23ndnd
2107-92-6Butanoic acidC4H8O288.19.40nd0.18nd
Aldehydes
3100-52-7BenzaldehydeC7H6O106.17.720.871.851.16
4122-78-1BenzeneacetaldehydeC8H8O120.110.790.120.860.57
52548-87-0(E)-2-OctenalC8H14O126.211.450.740.162.80
64432-63-7AtropaldehydeC9H8O132.114.94ndnd0.06
718829-56-6trans-2-NonenalC9H16O140.215.16ndnd0.05
8112-31-2DecanalC10H20O156.217.160.02nd0.05
95910-87-2(E,E)-2,4-NonadienalC9H14O138.217.49ndnd0.12
104411-89-62-Phenylbut-2-enalC10H10O146.119.33nd0.19nd
1125152-84-5(E,E)-2,4-DecadienalC10H16O152.220.82nd0.210.09
1213019-16-42-Butyl-2-octenalC12H22O152.222.090.65nd
Alcohols
13111-27-31-HexanolC6H14O102.14.74ndnd0.15
143391-86-41-Octen-3-olC8H16O128.28.7931.410.7768.67
15589-98-03-OctanolC8H18O130.29.480.06nd16.38
1618185-81-43-Octen-1-olC8H16O128.211.77nd0.59nd
1718409-17-1(E)-2-Octen-1-olC8H16O128.211.970.280.443.33
18111-87-51-OctanolC8H18O130.212.050.020.750.94
1934995-77-2trans-Furan linalool oxideC10H18O2170.212.450.250.73nd
20818-81-52-Methyl-1-octanolC9H20O144.212.650.290.50nd
213913-02-82-Butyl-1-octanolC12H26O186.320.35nd0.07nd
2240716-66-3NerolidolC15H26O222.325.610.070.28nd
Esters
23106-70-7Methyl hexanoateC7H14O2130.16.430.11nd0.01
24108-29-2γ-ValerolactoneC5H8O2100.17.340.240.25nd
25637-65-0Tetrahydrofurfuryl propionateC8H14O3158.210.850.100.160.07
26695-06-75-Ethyloxolan-2-oneC6H10O2114.111.080.10ndnd
27111-11-5Caprylic acid methyl esterC9H18O2158.213.85ndnd0.17
287367-81-9Methyl oct-2-enoateC9H16O2156.215.590.320.270.74
29959067-41-52-Ethylhexyl hexyl sulfiteC14H30O3S278.420.24nd0.10nd
301191-02-2Methyl dec-4-enoateC11H20O2184.220.510.16nd0.54
31103-26-4Methyl cinnamateC10H10O2162.222.4519.9352.762.07
324493-42-9Methyl 2E,4Z-decadienoateC11H18O2182.222.62nd0.430.06
3379837-88-0Methyl (Z)-dodec-5-enoateC13H24O2212.324.80nd0.540.18
3484-69-5Diisobutyl phthalateC16H22O4278.330.100.02nd0.01
3584-74-2Dibutyl phthalateC16H22O4278.331.670.030.09nd
Ketones
36497-23-42(5H)-FuranoneC4H4O284.05.970.756.38nd
374312-99-61-Octen-3-oneC8H14O126.18.361.950.72nd
382918-13-01-Hepten-3-oneC7H12O112.18.54nd0.140.24
39106-68-33-OctanoneC8H16O128.28.66nd0.84nd
4014705-50-12,2,5-Trimethyl-3-hexanoneC9H18O142.29.20nd0.35nd
411073-11-6Lavender lactoneC7H10O2126.110.461.080.66nd
4218402-82-93-Octen-2-oneC8H14O126.210.640.460.19nd
4398-86-2AcetophenoneC8H8O120.111.67ndnd0.02
443508-78-93-Allylpentane-2,4-dioneC8H12O2140.111.74nd0.380.05
45693-54-92-DecanoneC10H20O156.216.450.290.260.01
46927-49-16-UndecanoneC11H22O170.219.470.080.44nd
47112-12-92-UndecanoneC11H22O170.320.130.260.530.08
Alkanes
485750-02-72-Cyclopropyl-butaneC7H1498.24.14ndnd0.01
492415-72-7Propyl-cyclopropaneC6H1284.14.650.23ndnd
505911-04-63-Methyl-nonaneC10H22142.28.120.040.09nd
51107-83-52-Methyl-pentaneC6H1486.19.61nd0.04nd
5217301-32-54,7-Dimethyl-undecaneC13H28184.311.25nd0.45nd
5316747-50-51-Ethyl-1-methyl-cyclopentaneC8H16112.211.36nd0.21nd
541120-21-4UndecaneC11H24156.313.023.75ndnd
554292-92-6Pentyl-cyclohexaneC11H22154.214.210.05ndnd
5671138-64-23-Methyl-undecaneC12H26168.314.52nd0.14nd
5762238-12-42,3,6-Trimethyl-decaneC13H28184.315.360.03ndnd
581502-38-1Methyl-cyclooctaneC9H18126.216.460.25ndnd
59112-40-3DodecaneC12H26170.316.884.143.80nd
60560-21-42,3,3-Trimethyl-pentaneC8H18114.219.210.010.05nd
615881-17-43-Ethyl-octaneC10H22142.219.580.020.63nd
62111-65-9OctaneC8H18114.220.391.451.070.01
6317301-33-64,8-Dimethyl-undecaneC13H28184.321.37nd0.28nd
6417312-57-13-Methyl-dodecaneC8H18184.321.830.040.07nd
651072-16-82,7-Dimethyl-octaneC10H22142.222.110.240.080.01
663891-98-32,6,10-Trimethyl-dodecaneC15H32212.422.240.09ndnd
67629-59-4TetradecaneC14H30198.322.790.960.320.11
6814905-56-72,6,10-TrimethyltetradecaneC17H36240.423.970.25ndnd
69629-62-9PentadecaneC15H32212.424.710.17nd0.01
7017302-01-13-Ethyl-3-methylheptaneC10H22142.225.27nd0.050.01
71563-16-63,3-Dimethyl-hexaneC8H18114.226.380.090.070.01
Olefins
721002-33-11,3-OctadieneC8H14110.13.290.63nd0.03
7319549-87-22,4-Dimethyl-1-hepteneC9H18126.23.46nd0.39nd
74694-87-1BenzocyclobuteneC8H8104.15.210.18nd0.48
7516746-86-42,3-Dimethyl-1-hexeneC8H16112.23.63nd0.080.04
7680-56-8α-PineneC10H16136.26.641.150.210.03
77690-92-6(3Z)-2,2-Dimethyl-3-hexeneC8H16112.27.600.100.330.12
7813269-52-8(E)-3-HexeneC6H1284.19.316.650.16nd
7961142-36-73-Ethyl-2-methyl-1,3-hexadieneC9H16124.210.38nd0.690.18
8013877-91-3OcimeneC10H16136.213.000.235.43nd
8156728-10-03,4,5-Trimethyl-1-hexeneC9H18126.213.460.180.660.01
8271138-64-23-Methylene-undecaneC12H24168.316.150.120.08nd
833650-28-0(+)-SativenC15H24204.323.050.75ndnd
8439863-73-5(±)-β-BarbateneC15H24204.323.7715.027.640.21
8530364-38-6Dehydro-ar-ioneneC15H22O172.227.04nd0.64nd
Aromatic hydrocarbons
86100-41-4EthylbenzeneC8H10106.14.260.04ndnd
8795-47-6o-XyleneC8H10106.14.540.24nd0.07
8899-87-6p-CymeneC10H14134.213.570.10ndnd
8991-20-3NaphthaleneC10H8128.115.960.23ndnd
9090-12-01-Methyl-naphthaleneC11H10142.120.090.340.16nd
91575-43-91,6-Dimethyl-naphthaleneC12H12156.223.120.06ndnd
9296-76-42,4-Di-tert-butylphenolC14H22O206.324.82nd0.22nd
Heterocycle compounds
93109-08-0Methyl-pyrazineC5H6N294.14.390.03ndnd
94123-32-02,5-Dimethyl-pyrazineC6H8N2108.15.990.112.63nd
953777-69-32-Pentyl-furanC9H14O138.28.840.36ndnd
95100-84-53-MethylanisoleC8H10O122.19.970.78nd0.04
971003-46-91,1-Dioxide-2-methylthiolaneC5H10O2S134.118.48nd0.07nd
98111150-30-23,5-Dimethyl-2-(3-methylbutyl)pyrazineC11H18N2178.220.64nd0.16nd
99113604-56-11,2,3-TrimethyldiaziridineC4H10N286.125.90nd0.02nd
Notes: nd = not detected.
Table 2. The unique and common VOCs in dry T. matsutake after different drying treatments.
Table 2. The unique and common VOCs in dry T. matsutake after different drying treatments.
Unique VOCsCommon VOCs
VFDHADFRESHVFD/HAD/FRESH
Ethylbenzene2,4-Dimethyl-1-heptene2-Cyclopropyl-butaneα-Pinene
2-Methylpyrazine3-Octanone1-Hexanol(3Z)-2,2-Dimethyl-3-hexene
Propyl cyclopropane2,2,5-Trimethyl-3-hexanoneAcetophenoneBenzaldehyde
3-Methyl-butanoic acidButanoic acidCaprylic acid methyl esterBenzeneacetaldehyde
2-Pentyl-furan2-Methyl-pentaneAtropaldehydeTetrahydrofurfuryl propionate
Lavender lactone4,7-Dimethyl-undecanetrans-2-Nonenal(E)-2-Octenal
Undecane1-Ethyl-1-methyl-cyclopentane(E,E)-2,4-Nonadienal(E)-2-Octen-1-ol
p-Cymene3-Octen-1-ol 1-Octanol
Pentyl-cyclohexane3-Methyl-undecane 3,4,5-Trimethylhexene
2,3,6-Trimethyl-decade1,1-Dioxide-2-methylthiolane (E)-2-Octenoic acid, methyl ester
Naphthalene2-Phenylbut-2-enal 2-Decanone
Methyl-cyclooctane2-Ethylhexyl hexyl sulfite 2-Undecanone
2-Butyl-2-octenal2-Butyl-1-octanol Octane
2,6,10-Trimethyl-dodecane3,5-Dimethyl-2-(3-methylbutyl)pyrazine 2,7-Dimethyl-octane
(+)-Sativen4,8-Dimethyl-undecane Methyl cinnamate
1,6-Dimethyl-naphthalene2,4-Di-tert-butylphenol Tetradecane
2,6,10-Trimethyltetradecane1,2,3-Trimethyldiaziridine (±)-β-Barbatene
Dehydro-ar-ionene 3,3-Dimethyl-Hexane
1-Octen-3-ol
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Zhang, F.; Lu, B.; He, X.; Yu, F. Flavor Variations in Precious Tricholoma matsutake under Different Drying Processes as Detected with HS-SPME-GC-MS. Foods 2024, 13, 2123. https://doi.org/10.3390/foods13132123

AMA Style

Zhang F, Lu B, He X, Yu F. Flavor Variations in Precious Tricholoma matsutake under Different Drying Processes as Detected with HS-SPME-GC-MS. Foods. 2024; 13(13):2123. https://doi.org/10.3390/foods13132123

Chicago/Turabian Style

Zhang, Fengming, Bin Lu, Xinhua He, and Fuqiang Yu. 2024. "Flavor Variations in Precious Tricholoma matsutake under Different Drying Processes as Detected with HS-SPME-GC-MS" Foods 13, no. 13: 2123. https://doi.org/10.3390/foods13132123

APA Style

Zhang, F., Lu, B., He, X., & Yu, F. (2024). Flavor Variations in Precious Tricholoma matsutake under Different Drying Processes as Detected with HS-SPME-GC-MS. Foods, 13(13), 2123. https://doi.org/10.3390/foods13132123

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