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Article

Probiotic Potential of a Folate-Producing Strain Latilactobacillus sakei LZ217 and Its Modulation Effects on Human Gut Microbiota

by 1,2, 1,2, 1,2, 1,2, 1,2, 2,* and 1,*
1
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
2
Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou 310018, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Leyuan Li, Malgorzata Muc-Wierzgon and Sandra Martin-Pelaez
Foods 2022, 11(2), 234; https://doi.org/10.3390/foods11020234
Received: 10 December 2021 / Revised: 11 January 2022 / Accepted: 13 January 2022 / Published: 16 January 2022
(This article belongs to the Topic Probiotics, Prebiotics and Postbiotics in Human Health)
Folate is a B-vitamin required for DNA synthesis, methylation, and cellular division, whose deficiencies are associated with various disorders and diseases. Currently, most folic acid used for fortification is synthesized chemically, causing undesirable side effects. However, using folate-producing probiotics is a viable option, which fortify folate in situ and regulate intestinal microbiota. In this study, the folate production potential of newly isolated strains from raw milk was analyzed by microbiological assay. Latilactobacillus sakei LZ217 showed the highest folate production in Folic Acid Assay Broth, 239.70 ± 0.03 ng/μL. The folate produced by LZ217 was identified as 5-methyltetrahydrofolate. LZ217 was tolerant to environmental stresses (temperature, pH, NaCl, and ethanol), and was resistant to gastrointestinal juices. Additionally, the in vitro effects of LZ217 on human gut microbiota were investigated by fecal slurry cultures. 16S rDNA gene sequencing indicated that fermented samples containing LZ217 significantly increased the abundance of phylum Firmicutes and genus Lactobacillus, Faecalibacterium, Ruminococcus 2, Butyricicoccus compared to not containing. Short-chain fatty acids (SCFAs) analysis revealed that LZ217 also increased the production of butyric acid by fermentation. Together, L. sakei LZ217 could be considered as a probiotic candidate to fortify folate and regulate intestinal microecology. View Full-Text
Keywords: folate; gut microbiota; Latilactobacillus sakei LZ217; probiotic potentials; tolerance folate; gut microbiota; Latilactobacillus sakei LZ217; probiotic potentials; tolerance
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MDPI and ACS Style

Liu, M.; Chen, Q.; Sun, Y.; Zeng, L.; Wu, H.; Gu, Q.; Li, P. Probiotic Potential of a Folate-Producing Strain Latilactobacillus sakei LZ217 and Its Modulation Effects on Human Gut Microbiota. Foods 2022, 11, 234. https://doi.org/10.3390/foods11020234

AMA Style

Liu M, Chen Q, Sun Y, Zeng L, Wu H, Gu Q, Li P. Probiotic Potential of a Folate-Producing Strain Latilactobacillus sakei LZ217 and Its Modulation Effects on Human Gut Microbiota. Foods. 2022; 11(2):234. https://doi.org/10.3390/foods11020234

Chicago/Turabian Style

Liu, Manman, Qingqing Chen, Yalian Sun, Lingzhou Zeng, Hongchen Wu, Qing Gu, and Ping Li. 2022. "Probiotic Potential of a Folate-Producing Strain Latilactobacillus sakei LZ217 and Its Modulation Effects on Human Gut Microbiota" Foods 11, no. 2: 234. https://doi.org/10.3390/foods11020234

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