Synergistic Action of Mild Heat and Essential Oil Treatments on Culturability and Viability of Escherichia coli ATCC 25922 Tested In Vitro and in Fruit Juice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strain and Inoculum Preparation
2.2. Bacterial Culturability and Viability Assays
2.2.1. Standard Plate Count Method
2.2.2. Flow Cytometry Analysis (FCM)
2.3. Statistical Analysis
3. Results and Discussion
3.1. Effect of Thermal Treatments on E. coli ATCC 25922
3.2. Effect of OEO Treatment
3.3. Effect of Antimicrobial Treatment in Synthetic Medium and Recovery Ability: Mild Heat-OEO
3.4. In Vitro Treatments Efficacy Assessment: Subpopulations Culturability
3.5. Effect of Antimicrobial Treatment in Fruit Juice and Recovery Ability: Mild Heat-OEO
3.5.1. Microbial Challenge Test in Fruit Juice at pH 3.8
3.5.2. Microbial Challenge Test in Fruit Juice at pH 7.0
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Culture-Based Method | Flow Cytometry (% of Events) * | Equivalent Alive Cell Content (Log AFU/mL) ** | ||||
---|---|---|---|---|---|---|---|
Log CFU/mL | Eq Log Reduction | Eq % of Dead Ells | Alive | Damaged | Dead | ||
Untreated sample | 7.44 ± 0.23 a | 0 | 0 | 98.90 ± 0.13 | 0.30 ± 0.08 | 0.80 ± 0.06 | 7.23 ± 0.02 a |
55 °C-30 min | 4.99 ± 0.90 a | −2.45 ± 0.90 | 98.68 ± 2.07 | 78.06 ± 0.88 | 18.37 ± 0.88 | 3.57 ± 0.09 | 7.05 ± 0.01 b |
60 °C-30 min | 3.96 ± 0.82 a | −3.48 ± 0.82 | 99.60 ± 0.16 | 33.14 ± 0.27 | 46.42 ± 0.60 | 20.44 ± 0.40 | 6.72 ± 0.01 b |
65 °C-30 min | 1.33 ± 1.24 a | −6.11 ± 1.24 | 99.99 ± 0.001 | 1.15 ± 0.18 | 34.33 ± 3.51 | 64.59 ± 3.43 | 5.25 ± 0.05 b |
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Di Gregorio, L.; Tchuenchieu, A.; Poscente, V.; Arioli, S.; Del Fiore, A.; Costanzo, M.; Giorgi, D.; Lucretti, S.; Bevivino, A. Synergistic Action of Mild Heat and Essential Oil Treatments on Culturability and Viability of Escherichia coli ATCC 25922 Tested In Vitro and in Fruit Juice. Foods 2022, 11, 1615. https://doi.org/10.3390/foods11111615
Di Gregorio L, Tchuenchieu A, Poscente V, Arioli S, Del Fiore A, Costanzo M, Giorgi D, Lucretti S, Bevivino A. Synergistic Action of Mild Heat and Essential Oil Treatments on Culturability and Viability of Escherichia coli ATCC 25922 Tested In Vitro and in Fruit Juice. Foods. 2022; 11(11):1615. https://doi.org/10.3390/foods11111615
Chicago/Turabian StyleDi Gregorio, Luciana, Alex Tchuenchieu, Valeria Poscente, Stefania Arioli, Antonella Del Fiore, Manuela Costanzo, Debora Giorgi, Sergio Lucretti, and Annamaria Bevivino. 2022. "Synergistic Action of Mild Heat and Essential Oil Treatments on Culturability and Viability of Escherichia coli ATCC 25922 Tested In Vitro and in Fruit Juice" Foods 11, no. 11: 1615. https://doi.org/10.3390/foods11111615