Risk Assessment and Evaluation of Analytical Method of Polycyclic Aromatic Hydrocarbons (PAHs) for Deep-Fat Fried Pork Products in Korea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Materials
2.2. Sample Preparation for Evaluation PAH4
2.3. Extraction and Clean-Up for Pretreatment
2.4. HPLC-FLD Analysis of PAH4
2.5. Identification and Quantification of PAH4
2.6. Validation of Analytical Method
2.7. Application of TEQ Concentration
2.8. Exposure Assessment
2.9. Risk Characterization
2.10. Statistical Analysis
3. Results and Discussion
3.1. Validation and Analytical Quality Assurance for PAHs Analysis
3.2. Comparison of PAHs Content in Deep-Fat Fried Pork Products according to the Frying Conditions
3.3. Exposure Assessment
3.4. Risk Characterization
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Instrument | Dionex U3000 HPLC | ||
---|---|---|---|
Column | ZORBAX Eclipse C18 Plus (4.6 mm × 250 mm, 5 μm) | ||
Wavelength | Excitation (nm) | Emission (nm) | |
0–20 min | 245 | 390 | |
20–60 min | 294 | 404 | |
Mobile phase | Acetonitrile | Water | |
0–20 min | 65 | 35 | |
20–60 min | 70 | 30 | |
Flow rate | 1.0 mL/min | ||
Temperature | 37 °C | ||
Injection volume | 10.0 μL |
PAHs | LOD (μg/kg) 1 | LOQ (μg/kg) 2 | Linearity (R2) 3 | Recovery (%) 4 |
---|---|---|---|---|
Benz[a]anthrancene | 0.14 | 0.42 | y = 0.0251x + 0.0043 R2 = 0.9989 | 99.9 ± 1.1 |
Chrysene | 0.10 | 0.32 | y = 0.0176x + 0.0217 R2 = 0.9976 | 95.5 ± 1.4 |
Benzo[b]fluoranthene | 0.16 | 0.50 | y = 0.0192x + 0.0060 R2 = 0.9975 | 88.0 ± 0.2 |
Benzo[a]pyrene | 0.18 | 0.55 | Y = 0.1340x − 0.0318 R2 = 0.9932 | 91.8 ± 1.4 |
Oil | Cooking Temperature (°C) | Time | PAHs (μg/kg) 1 | TEQBaP (μg/kg/day) | |||||
---|---|---|---|---|---|---|---|---|---|
(min) | BaA | CHR | BbF | BaP | PAH4 | BaP | PAH4 | ||
0 | <LOQ 2 | <LOQ | <LOQ | 0.38 ± 0.26 | 0.38 ± 0.26 | 0.38 | 0.38 | ||
Soybean | 160 | 3 | <LOQ | <LOQ | <LOQ | 0.86 ± 0.14 | 0.86 ± 0.14 | 0.86 | 0.86 |
6 | <LOQ | <LOQ | <LOQ | 1.59 ± 0.52 | 1.59 ± 0.52 | 1.59 | 1.59 | ||
9 | 0.55 ± 0.07 | <LOQ | <LOQ | 2.66 ± 0.31 | 3.21 ± 0.37 | 2.66 | 2.71 | ||
180 | 3 | <LOQ | <LOQ | <LOQ | 1.50 ± 0.15 | 1.50 ± 0.15 | 1.50 | 1.50 | |
6 | 0.14 ± 0.24 | <LOQ | <LOQ | 2.42 ± 0.14 | 2.56 ± 0.33 | 2.42 | 2.44 | ||
9 | 0.71 ± 0.07 | <LOQ | <LOQ | 3.50 ± 0.56 | 4.21 ± 0.50 | 3.50 | 3.57 | ||
200 | 3 | <LOQ | <LOQ | <LOQ | 2.66 ± 0.32 | 2.66 ± 0.32 | 2.66 | 2.66 | |
6 | 0.17 ± 0.29 | <LOQ | <LOQ | 3.59 ± 0.12 | 3.76 ± 0.33 | 3.59 | 3.61 | ||
9 | 0.86 ± 0.09 | <LOQ | <LOQ | 5.95 ± 2.01 | 6.81 ± 2.00 | 5.95 | 6.03 | ||
Canola | 160 | 3 | <LOQ | <LOQ | <LOQ | 1.03 ± 0.16 | 1.03 ± 0.16 | 1.03 | 1.03 |
6 | <LOQ | <LOQ | <LOQ | 1.63 ± 0.12 | 1.63 ± 0.12 | 1.63 | 1.63 | ||
9 | <LOQ | 0.14 ± 0.25 | <LOQ | 3.72 ± 0.58 | 3.86 ± 0.67 | 3.72 | 3.72 | ||
180 | 3 | <LOQ | <LOQ | <LOQ | 1.43 ± 0.09 | 1.43 ± 0.09 | 1.43 | 1.43 | |
6 | <LOQ | <LOQ | <LOQ | 1.99 ± 0.36 | 1.99 ± 0.36 | 1.99 | 1.99 | ||
9 | 0.19 ± 0.32 | 0.18 ± 0.31 | <LOQ | 2.54 ± 0.42 | 2.90 ± 0.14 | 2.54 | 2.56 | ||
200 | 3 | <LOQ | <LOQ | <LOQ | 1.71 ± 0.16 | 1.71 ± 0.16 | 1.71 | 1.71 | |
6 | <LOQ | 0.15 ± 0.27 | <LOQ | 2.95 ± 0.32 | 3.10 ± 0.59 | 2.95 | 2.95 | ||
9 | 0.23 ± 0.39 | 0.22 ± 0.38 | <LOQ | 5.06 ± 1.22 | 5.50 ± 0.88 | 5.06 | 5.08 | ||
Grape seed | 160 | 3 | <LOQ | <LOQ | <LOQ | 1.06 ± 0.09 | 1.06 ± 0.09 | 1.06 | 1.06 |
6 | <LOQ | 0.20 ± 0.34 | <LOQ | 1.49 ± 0.14 | 1.69 ± 0.48 | 1.49 | 1.49 | ||
9 | <LOQ | 0.22 ± 0.39 | <LOQ | 2.52 ± 0.03 | 2.74 ± 0.40 | 2.52 | 2.52 | ||
180 | 3 | <LOQ | <LOQ | <LOQ | 1.40 ± 0.17 | 1.40 ± 0.17 | 1.40 | 1.40 | |
6 | <LOQ | 0.2 ± 0.35 | <LOQ | 2.01 ± 0.07 | 2.21 ± 0.36 | 2.01 | 2.01 | ||
9 | <LOQ | 0.24 ± 0.42 | <LOQ | 3.80 ± 1.61 | 4.04 ± 2.03 | 3.80 | 3.80 | ||
200 | 3 | <LOQ | 0.16 ± 0.28 | <LOQ | 1.91 ± 0.48 | 2.07 ± 0.55 | 1.91 | 1.91 | |
6 | <LOQ | 0.23 ± 0.4 | <LOQ | 2.95 ± 0.32 | 3.18 ± 0.67 | 2.95 | 2.95 | ||
9 | 0.27 ± 0.47 | 0.80 ± 0.78 | <LOQ | 6.83 ± 1.33 | 7.90 ± 2.46 | 6.83 | 6.86 | ||
Sunflower | 160 | 3 | <LOQ | <LOQ | <LOQ | 1.09 ± 0.07 | 1.09 ± 0.07 | 1.09 | 1.09 |
6 | <LOQ | <LOQ | <LOQ | 1.33 ± 0.20 | 1.33 ± 0.20 | 1.33 | 1.33 | ||
9 | <LOQ | 0.19 ± 0.32 | <LOQ | 2.44 ± 1.21 | 2.63 ± 1.11 | 2.44 | 2.45 | ||
180 | 3 | <LOQ | 0.16 ± 0.28 | <LOQ | 1.31 ± 0.07 | 1.47 ± 0.34 | 1.31 | 1.31 | |
6 | <LOQ | 0.33 ± 0.28 | <LOQ | 2.63 ± 0.05 | 2.95 ± 0.25 | 2.63 | 2.63 | ||
9 | <LOQ | 0.43 ± 0.38 | <LOQ | 4.41 ± 1.56 | 4.84 ± 1.79 | 4.41 | 4.42 | ||
200 | 3 | <LOQ | 0.17 ± 0.29 | <LOQ | 2.21 ± 0.59 | 2.37 ± 0.66 | 2.21 | 2.21 | |
6 | <LOQ | 0.45 ± 0.41 | <LOQ | 3.42 ± 0.45 | 3.87 ± 0.68 | 3.42 | 3.42 | ||
9 | <LOQ | 0.37 ± 0.64 | <LOQ | 6.81 ± 2.48 | 7.17 ± 3.02 | 6.81 | 6.81 |
Age | Soybean Oil | Canola Oil | Grape Seed Oil | Sunflower Oil | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
BaP (μg/kg) | PAH4 (μg/kg) | BaP (μg/kg) | PAH4 (μg/kg) | BaP (μg/kg) | PAH4 (μg/kg) | BaP (μg/kg) | PAH4 (μg/kg) | |||||||||
Mean | 95th | Mean | 95th | Mean | 95th | Mean | 95th | Mean | 95th | Mean | 95th | Mean | 95th | Mean | 95th | |
>65 | 0.32 | 2.03 | 0.33 | 2.06 | 0.28 | 1.72 | 0.28 | 1.73 | 0.37 | 2.33 | 0.37 | 2.34 | 0.37 | 2.32 | 0.37 | 2.32 |
50–64 | 0.38 | 1.90 | 0.39 | 1.93 | 0.33 | 1.62 | 0.33 | 1.62 | 0.44 | 2.18 | 0.44 | 2.19 | 0.44 | 2.18 | 0.44 | 2.18 |
30–49 | 0.77 | 3.66 | 0.78 | 3.72 | 0.66 | 3.11 | 0.66 | 3.13 | 0.89 | 4.20 | 0.89 | 4.23 | 0.89 | 4.19 | 0.89 | 4.19 |
19–29 | 0.53 | 2.64 | 0.54 | 2.67 | 0.45 | 2.24 | 0.45 | 2.25 | 0.61 | 3.03 | 0.61 | 3.04 | 0.60 | 3.02 | 0.61 | 3.02 |
12–18 | 0.36 | 1.61 | 0.37 | 1.63 | 0.31 | 1.37 | 0.31 | 1.37 | 0.42 | 1.85 | 0.42 | 1.86 | 0.42 | 1.84 | 0.42 | 1.84 |
6–11 | 0.32 | 1.36 | 0.33 | 1.38 | 0.28 | 1.16 | 0.28 | 1.17 | 0.37 | 1.57 | 0.38 | 1.57 | 0.37 | 1.56 | 0.37 | 1.56 |
3–5 | 0.18 | 0.77 | 0.18 | 0.78 | 0.15 | 0.65 | 0.16 | 0.66 | 0.21 | 0.88 | 0.21 | 0.88 | 0.21 | 0.88 | 0.21 | 0.88 |
1–2 | 0.09 | 0.52 | 0.09 | 0.53 | 0.08 | 0.44 | 0.08 | 0.44 | 0.11 | 0.60 | 0.11 | 0.60 | 0.11 | 0.60 | 0.11 | 0.60 |
Age | 3 min | 6 min | 9 min | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | |||||||
BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | |
>65 | 9.36 ×105 | 4.55 ×106 | 1.50 ×105 | 7.29 ×105 | 6.10 ×105 | 2.96 ×106 | 9.77 ×104 | 4.74 ×105 | 3.50 ×105 | 1.69 ×106 | 5.61 ×104 | 2.71 ×105 |
50–64 | 7.90 ×105 | 3.77 ×106 | 1.60 ×105 | 7.63 ×105 | 5.15 ×105 | 2.38 ×106 | 1.04 ×105 | 4.81 ×105 | 2.96 ×105 | 1.29 ×106 | 5.99 ×104 | 2.61 ×105 |
30–49 | 3.93 ×105 | 1.91 ×106 | 8.30 ×104 | 3.96 ×105 | 2.56 ×105 | 1.24 ×106 | 5.41 ×104 | 2.50 ×105 | 1.47 ×105 | 7.10 ×105 | 3.11 ×104 | 1.36 ×105 |
19–29 | 5.76 ×105 | 2.75 ×106 | 1.15 ×106 | 5.50 ×105 | 3.75 ×105 | 1.73 ×106 | 7.51 ×104 | 3.47 ×105 | 2.15 ×105 | 9.40 ×105 | 4.32 ×104 | 1.88 ×105 |
12–18 | 8.38 ×105 | 4.07 ×106 | 1.89 ×105 | 9.19 ×105 | 5.46 ×105 | 2.65 ×106 | 1.23 ×105 | 5.98 ×105 | 3.14 ×105 | 1.51 ×106 | 7.08 ×104 | 3.42 ×105 |
6–11 | 9.36 ×105 | 4.54 ×106 | 2.22 ×105 | 1.08 ×105 | 6.10 ×105 | 2.96 ×106 | 1.45 ×105 | 7.04 ×105 | 3.50 ×105 | 1.69 ×106 | 8.34 ×104 | 4.02 ×105 |
3–5 | 1.67 ×106 | 8.11 ×106 | 3.97 ×105 | 1.93 ×106 | 1.09 ×107 | 5.28 ×106 | 2.58 ×105 | 1.25 ×106 | 6.25 ×105 | 3.01 ×106 | 1.48 ×105 | 7.16 ×105 |
1–2 | 3.28 ×106 | 1.59 ×107 | 5.84 ×105 | 2.84 ×106 | 2.14 ×107 | 1.04 ×107 | 3.80 ×105 | 1.84 ×106 | 1.23 ×106 | 5.92 ×106 | 2.19 ×105 | 1.05 ×106 |
Age | 160 °C | 180 °C | 200 °C | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | Average Dietary Exposure (μg-TEQBaP/kg/day) | 95th Percentile Dietary Exposure (μg-TEQBaP/kg/day) | |||||||
BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | BaP | PAH4 | |
>65 | 8.66 ×105 | 4.20 ×106 | 1.39 ×105 | 6.73 ×105 | 6.20 ×105 | 3.00 ×106 | 3.00 ×104 | 9.94 ×105 | 4.10 ×105 | 1.99 ×106 | 6.58 ×104 | 3.19 ×105 |
50–64 | 7.31 ×105 | 3.46 ×106 | 1.48 ×105 | 7.00 ×105 | 5.23 ×105 | 2.40 ×106 | 1.06 ×105 | 4.86 ×105 | 3.46 ×105 | 1.58 ×106 | 7.01 ×104 | 3.21 ×105 |
30–49 | 3.64 ×105 | 1.76 ×106 | 7.68 ×104 | 3.63 ×105 | 2.60 ×105 | 1.26 ×106 | 5.50 ×104 | 2.52 ×105 | 1.72 ×105 | 8.36 ×105 | 3.64 ×104 | 1.66 ×105 |
19–29 | 5.32 ×105 | 2.52 ×106 | 1.07 ×105 | 5.05 ×105 | 3.81 ×105 | 1.75 ×106 | 7.64 ×104 | 3.50 ×105 | 2.52 ×105 | 1.15 ×106 | 5.06 ×104 | 2.31 ×105 |
12–18 | 7.75 ×105 | 3.76 ×106 | 1.75 ×105 | 8.49 ×105 | 5.55 ×105 | 2.69 ×106 | 1.25 ×105 | 6.07 ×105 | 3.67 ×105 | 1.78 ×106 | 8.29 ×104 | 4.02 ×105 |
6–11 | 8.66 ×105 | 4.20 ×106 | 2.06 ×105 | 1.00 ×106 | 6.20 ×105 | 3.00 ×106 | 1.48 ×105 | 7.15 ×105 | 4.10 ×105 | 1.99 ×106 | 9.78 ×104 | 4.74 ×105 |
3–5 | 1.54 ×106 | 7.49 ×106 | 3.67 ×105 | 1.78 ×106 | 1.11 ×106 | 5.36 ×106 | 2.63 ×105 | 1.27 ×106 | 7.32 ×105 | 3.55 ×106 | 1.74 ×105 | 8.43 ×105 |
1–2 | 3.03 ×106 | 1.47 ×107 | 5.40 ×105 | 2.62 ×106 | 2.17 ×106 | 1.04 ×107 | 3.87 ×105 | 1.88 ×106 | 1.44 ×106 | 6.97 ×106 | 2.56 ×105 | 1.24 ×106 |
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Kim, S.Y.; Shin, H.W.; Kim, G.H.; Kim, Y.-Y.; Kang, M.-J.; Shin, H.-S. Risk Assessment and Evaluation of Analytical Method of Polycyclic Aromatic Hydrocarbons (PAHs) for Deep-Fat Fried Pork Products in Korea. Foods 2022, 11, 1618. https://doi.org/10.3390/foods11111618
Kim SY, Shin HW, Kim GH, Kim Y-Y, Kang M-J, Shin H-S. Risk Assessment and Evaluation of Analytical Method of Polycyclic Aromatic Hydrocarbons (PAHs) for Deep-Fat Fried Pork Products in Korea. Foods. 2022; 11(11):1618. https://doi.org/10.3390/foods11111618
Chicago/Turabian StyleKim, Seo Yeon, Hye Won Shin, Geon Hee Kim, Yong-Yeon Kim, Min-Jae Kang, and Han-Seung Shin. 2022. "Risk Assessment and Evaluation of Analytical Method of Polycyclic Aromatic Hydrocarbons (PAHs) for Deep-Fat Fried Pork Products in Korea" Foods 11, no. 11: 1618. https://doi.org/10.3390/foods11111618