Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Bacterial Suspension
2.2. Animal Experiments
2.3. Measurement of Disease Activity Index
2.4. Measurement of Cell Cytokines and Antioxidant System in the Colon
2.5. Histological Analysis
2.6. Immunohistochemical Analysis
2.7. Quantitative RT-PCR
2.8. SCFA Analysis
2.9. Bioinformatics Analysis of 16S rRNA Gene Sequences
2.10. Statistical Analysis
3. Results
3.1. Influence of B. pseudolongum Intervention on Body Weight and Colon Morphology in Colitis Mice
3.2. B. pseudolongum Intervention Relieves Colonic Cell Cytokines and Oxidative Stress in Colitis Mice
3.3. B. pseudolongum Intervention Attenuates Gut Barrier Integrity in Colitis Mice
3.4. B. pseudolongum Intervention Modulates the PPARγ/STAT3 Pathway
3.5. B. pseudolongum Alters Intestinal Microbiota Composition
3.6. Influences of B. pseudolongum on SCFA Levels in Mice with DSS Treatment
3.7. The Associations between Colitis-Related Parameters and Key Microbiota
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guo, W.; Mao, B.; Cui, S.; Tang, X.; Zhang, Q.; Zhao, J.; Zhang, H. Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota. Foods 2022, 11, 1551. https://doi.org/10.3390/foods11111551
Guo W, Mao B, Cui S, Tang X, Zhang Q, Zhao J, Zhang H. Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota. Foods. 2022; 11(11):1551. https://doi.org/10.3390/foods11111551
Chicago/Turabian StyleGuo, Weiling, Bingyong Mao, Shumao Cui, Xin Tang, Qiuxiang Zhang, Jianxin Zhao, and Hao Zhang. 2022. "Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota" Foods 11, no. 11: 1551. https://doi.org/10.3390/foods11111551