Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000–2005 and 2019–2020
Abstract
:1. Introduction
2. Materials and Methods
2.1. Researched Sources from Meat Products in 2019
2.2. Culturing Conditions of Bacterial Strains
2.3. Antibiotic Resistance Assays
2.4. L. monocytogenes Virulence in HEp-2 Cells
2.5. Multiplex PCR Assay to Serotype L. monocytogenes
2.6. PCR
2.7. Sequencing of PCR Products
2.8. Sequence Analysis
2.9. Calculation of Diversity Indexes
2.10. Statistics
3. Results
3.1. Phylogenetic Characterization of L. monocytogenes Strains Isolated from Raw Food Products in 2001–2005 and 2019–2020
3.2. Occurrence of Worldwide Distributed Epidemic Clones among Studied Strains
3.3. MvLST Typing Did Not Increase Resolution of the MLST Scheme but Showed Similarity of Invasion Factors InlA and InlB in Strains Belonging to Distinct CCs
3.4. Invasion of L. monocytogenes into Human HEp-2 Cells
3.5. Antibiotics Resistance of L. monocytogenes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Psareva, E.K.; Liskova, E.A.; Razheva, I.V.; Yushina, Y.K.; Grudistova, M.A.; Gladkova, N.A.; Potemkin, E.A.; Zhurilov, P.A.; Sokolova, E.V.; Andriyanov, P.A.; et al. Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000–2005 and 2019–2020. Foods 2021, 10, 2790. https://doi.org/10.3390/foods10112790
Psareva EK, Liskova EA, Razheva IV, Yushina YK, Grudistova MA, Gladkova NA, Potemkin EA, Zhurilov PA, Sokolova EV, Andriyanov PA, et al. Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000–2005 and 2019–2020. Foods. 2021; 10(11):2790. https://doi.org/10.3390/foods10112790
Chicago/Turabian StylePsareva, Ekaterina K., Elena A. Liskova, Irina V. Razheva, Yulia K. Yushina, Maria A. Grudistova, Nadezda A. Gladkova, Eugene A. Potemkin, Pavel A. Zhurilov, Elena V. Sokolova, Pavel A. Andriyanov, and et al. 2021. "Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000–2005 and 2019–2020" Foods 10, no. 11: 2790. https://doi.org/10.3390/foods10112790
APA StylePsareva, E. K., Liskova, E. A., Razheva, I. V., Yushina, Y. K., Grudistova, M. A., Gladkova, N. A., Potemkin, E. A., Zhurilov, P. A., Sokolova, E. V., Andriyanov, P. A., Voronina, O. L., Kolbasov, D. V., & Ermolaeva, S. A. (2021). Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000–2005 and 2019–2020. Foods, 10(11), 2790. https://doi.org/10.3390/foods10112790