Analysis of the Chemical Profiles and Anti-S. aureus Activities of Essential Oils Extracted from Different Parts of Three Oregano Cultivars
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Bacterial Strains, and Standards
2.2. Extraction of OEOs
2.3. Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of OEOs
2.4. Evaluation of Antibacterial Activity
2.4.1. Diameter of the Inhibitory Zone (DIZ) Assays
2.4.2. Minimum Inhibitory Concentration (MIC) Assays
2.5. Statistical Analysis
3. Results and Discussion
3.1. Morphological Observations
3.2. Chemical Composition of OEOs
3.3. Characterization of Common and Unique OEO Components
3.4. Multivariate Statistical Analysis of EO Chemical Profiles
3.5. Antibacterial Activity of OEOs
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compounds | RI | Ovhi | Ovhl | Ovhs | Omhi | Omhl | Omhs | Ovli | Ovll |
---|---|---|---|---|---|---|---|---|---|
α-Thujene | 932 | 0.06 ± 0.06 | 0.08 ± 0.03 | 0.19 ± 0.26 | 1.01 ± 0.4 | 0.97 ± 0.97 | - | - | 0.33 ± 0.23 |
Sabinene | 973 | - | - | - | 0.29 ± 0.26 | 0.88 ± 0.6 | 0.05 ± 0.01 | 0.15 ± 0.14 | 1.65 ± 1.35 |
β-Myrcene | 991 | 0.08 ± 0.01 | 0.09 ± 0.04 | 0.13 ± 0.11 | 0.24 ± 0.04 | 0.48 ± 0.04 | - | - | 0.87 ± 0.65 |
α-Phellandrene | 1006 | - | - | 0.07 ± 0.08 | 0.49 ± 0.1 | 0.37 ± 0.15 | - | - | - |
α-Terpinene | 1018 | 0.39 ± 0.05 | 0.07 ± 0.13 | 0.5 ± 0.43 | 11.27 ± 0.78 | 8.74 ± 1.54 | 0.6 ± 0.07 | 0.68 ± 0.13 | 2.92 ± 1.99 |
p-Cymene | 1026 | 2.16 ± 0.3 | 3.37 ± 0.51 | 5.65 ± 4.58 | 0.79 ± 0.01 | 2.02 ± 0.15 | 0.51 ± 0.02 | 0.78 ± 0.04 | 5.72 ± 3.54 |
β-Phellandrene | 1030 | 0.04 ± 0.06 | 0.06 ± 0.06 | 0.1 ± 0.17 | 0.25 ± 0.25 | 1.3 ± 1.26 | 0.04 ± 0.07 | 0.3 ± 0.15 | 1.67 ± 1.08 |
Eucalyptol | - | - | - | - | - | - | - | 0.18 ± 0.16 | |
cis-β-Ocimene | 1038 | - | - | 0.04 ± 0.04 | - | - | 0.04 ± 0.04 | 0.14 ± 0 | 3.47 ± 2.16 |
γ-Terpinene | 1060 | 0.77 ± 0.2 | 0.23 ± 0.06 | 0.79 ± 0.56 | 13.79 ± 0.99 | 13.3 ± 0.16 | 1.69 ± 0.11 | 2.62 ± 0.12 | 12.28 ± 6.59 |
trans-Sabinene hydrate | 1069 | 0.3 ± 0.12 | 0.4 ± 0.08 | 1.02 ± 0.1 | 6.54 ± 3.76 | 14.6 ± 0.5 | 9.36 ± 0.14 | - | - |
Terpinolene | 1091 | 0.12 ± 0.03 | 0.26 ± 0.15 | 0.19 ± 0.17 | - | 1.8 ± 1.57 | 0.41 ± 0.02 | 0.15 ± 0.13 | 0.23 ± 0.21 |
Linalool | - | - | - | - | - | - | 0.21 ± 0.04 | 0.98 ± 0.28 | |
cis-2-p-Menthen-1-ol | 1124 | 0.09 ± 0.03 | 0.08 ± 0.02 | 0.23 ± 0.07 | 2.28 ± 0.16 | 2.58 ± 0.18 | 2.46 ± 0.12 | - | 0.27 ± 0.22 |
endo-Borneol | 1170 | 0.17 ± 0.01 | 0.25 ± 0.02 | 0.53 ± 0.12 | 0.18 ± 0 | 0.19 ± 0.01 | 0.88 ± 0.04 | - | - |
Terpinen-4-ol | 1181 | 1.36 ± 0.32 | 1.08 ± 0.11 | 3.64 ± 0.91 | 25.05 ± 1.55 | 22.13 ± 0.98 | 21.42 ± 0.76 | 2.17 ± 0.1 | 7.21 ± 1.32 |
p-Cymen-8-ol | 1188 | - | 0.17 ± 0.01 | 0.14 ± 0.04 | - | - | - | - | - |
α-Terpineol | 1194 | 0.19 ± 0.04 | 0.17 ± 0.02 | 0.78 ± 0.24 | 4.71 ± 0.08 | 4.15 ± 0.14 | 4.77 ± 0.08 | 0.75 ± 0.09 | 1.58 ± 0.21 |
trans-Dihydrocarvone | 1201 | 0.24 ± 0.05 | 0.31 ± 0.04 | 0.33 ± 0.06 | 0.1 ± 0 | 0.09 ± 0.02 | 0.32 ± 0.01 | - | - |
trans-Piperitol | 1211 | - | - | 0.12 ± 0.06 | 0.72 ± 0.04 | 0.78 ± 0.04 | 0.97 ± 0.01 | - | - |
Carveol | 1223 | - | 0.06 ± 0.02 | 0.12 ± 0.05 | - | - | 0.13 ± 0.01 | - | - |
Carvacrol methyl ether | 1248 | 0.63 ± 0.1 | 0.99 ± 0.18 | 0.87 ± 0.07 | 0.05 ± 0 | 0.05 ± 0.01 | 0.6 ± 0.03 | - | 0.17 ± 0.03 |
Linalyl acetate | 1259 | - | - | - | 0.5 ± 0.18 | 1.14 ± 0.14 | 0.2 ± 0.09 | - | 0.17 ± 0.15 |
Thymol | 1294 | 0.1 ± 0.17 | 0.23 ± 0.2 | 0.21 ± 0.37 | 18.74 ± 0.62 | 14.31 ± 0.75 | 18.72 ± 0.08 | 0.45 ± 0.4 | 2.49 ± 0.51 |
Carvacrol | 1304 | 90.28 ± 1.5 | 86.03 ± 0.66 | 79.31 ± 5.83 | 7.01 ± 0.32 | 5.25 ± 0.28 | 27.73 ± 0.94 | 0.67 ± 0.33 | 2 ± 1.81 |
δ-EIemene | - | - | - | - | - | - | 0.83 ± 0.04 | 0.43 ± 0.11 | |
Nerol acetate | 1368 | - | - | - | 0.09 ± 0.1 | 0.15 ± 0.09 | 0.26 ± 0.13 | - | - |
Copaene | 1382 | 0.05 ± 0.04 | 0.07 ± 0.01 | 0.05 ± 0.01 | 0.11 ± 0.1 | - | 0.12 ± 0.1 | 0.63 ± 0.06 | 0.33 ± 0.13 |
β-Bourbonene | 1393 | 0.03 ± 0.02 | 0.15 ± 0.02 | 0.05 ± 0.04 | - | 0.05 ± 0.01 | 0.15 ± 0.02 | 0.1 ± 0.08 | 0.85 ± 0.19 |
β-Elemen | - | - | - | - | - | - | 0.44 ± 0.01 | 0.48 ± 0.1 | |
α-Gurjunene | - | - | - | - | - | - | 0.18 ± 0.04 | - | |
β-Caryophyllene | 1428 | 1.36 ± 0.17 | 1.95 ± 0.18 | 0.93 ± 0.23 | 2.69 ± 0.05 | 2.03 ± 0.13 | 2.42 ± 0.1 | 13.99 ± 0.18 | 10.51 ± 2.41 |
Alloaromadendrene | - | - | - | - | - | - | 0.3 ± 0.09 | 0.35 ± 0.28 | |
Humulene | 1462 | 0.16 ± 0.02 | 0.23 ± 0.02 | 0.11 ± 0.02 | 0.24 ± 0 | 0.19 ± 0.02 | 0.26 ± 0.02 | 3 ± 0.09 | 1.95 ± 0.53 |
epi-β-Caryophyllene | - | - | - | - | - | - | 1.48 ± 0.08 | 0.55 ± 0.46 | |
Cadina-1(6),4-diene | - | - | - | - | - | - | 0.33 ± 0.03 | 0.09 ± 0.09 | |
Germacrene D | 1484 | 0.41 ± 0.03 | 0.66 ± 0.07 | 0.32 ± 0.17 | 1.34 ± 0.11 | 0.92 ± 0.09 | 0.76 ± 0.03 | 11.13 ± 0.65 | 11.26 ± 2.56 |
γ-Muurolene | - | - | - | - | - | - | 0.85 ± 0.17 | 1.01 ± 0.41 | |
α-Bulnesene | 1502 | - | 0.11 ± 0.1 | 0.06 ± 0.05 | 0.06 ± 0.04 | - | - | - | - |
Elixene | 1505 | - | - | - | 0.79 ± 0.04 | 0.56 ± 0.03 | 0.34 ± 0.12 | 25.27 ± 0.93 | 13.69 ± 2.67 |
α-Muurolene | 1507 | 0.04 ± 0.04 | 0.1 ± 0 | 0.07 ± 0.07 | - | - | - | - | - |
α-Farnesene | - | - | - | - | - | - | 3.39 ± 0.13 | 0.98 ± 0.91 | |
β-Bisabolene | 1514 | 0.19 ± 0.01 | 0.34 ± 0.02 | 0.72 ± 0.08 | 0.32 ± 0.01 | 0.29 ± 0.03 | 0.72 ± 0.05 | - | - |
γ-Cadinene | - | - | - | - | - | - | 2.33 ± 0.1 | 0.77 ± 0.29 | |
δ-Cadinene | 1530 | 0.38 ± 0.04 | 0.51 ± 0.02 | 0.36 ± 0.06 | 0.11 ± 0.01 | 0.05 ± 0.01 | 0.24 ± 0.21 | 7.75 ± 0.11 | 2.35 ± 0.88 |
1,4-Cadinadiene | - | - | - | - | - | - | 0.21 ± 0.02 | - | |
α-Cadinene | - | - | - | - | - | - | 0.45 ± 0.01 | - | |
Elemol | - | - | - | - | - | - | - | 0.43 ± 0.28 | |
Globulol | - | - | - | - | - | - | 1.88 ± 0.36 | 0.74 ± 1.19 | |
Spathulenol | 1586 | - | - | 0.08 ± 0 | - | 0.06 ± 0.05 | 0.73 ± 0.19 | 4.6 ± 0.28 | 3.47 ± 1.62 |
Caryophyllene oxide | 1592 | 0.17 ± 0.04 | 0.91 ± 0.11 | 1.22 ± 0.12 | - | 0.09 ± 0.01 | 1.15 ± 0.21 | 0.16 ± 0.01 | 0.64 ± 0.61 |
Viridiflorol | - | - | - | - | - | - | 1.05 ± 0.22 | 0.51 ± 0.3 | |
Rosifoliol | - | - | - | - | - | - | 0.33 ± 0.08 | - | |
Epicubenol | - | - | - | - | - | - | 0.25 ± 0.01 | 0.12 ± 0.14 | |
γ-Eudesmol | - | - | - | - | - | - | 0.3 ± 0.02 | 0.29 ± 0.21 | |
δ-Cadinol | 1648 | - | - | - | - | - | 0.2 ± 0.12 | 4.25 ± 0.16 | 1.09 ± 0.61 |
β-Eudesmol | - | - | - | - | - | - | - | 0.4 ± 0.32 | |
α-Cadinol | - | - | - | - | - | - | 4.43 ± 0.01 | 1.42 ± 0.86 | |
Total | 99.77 ± 0.13 | 98.98 ± 0.24 | 98.93 ± 0.31 | 99.75 ± 0.16 | 99.54 ± 0.55 | 98.25 ± 0.54 | 98.96 ± 0.32 | 98.95 ± 0.7 | |
EO yields | 5.83 ± 0.15 | 2.23 ± 0.06 | 0.2 ± 0 | 2.3 ± 0.1 | 1.03 ± 0.12 | 0.1 ± 0 | 0.27 ± 0.06 | 0.1 ± 0 |
Bacteria | Concentrations of OEO (mg/mL) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0.0625 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | 64 | 128 | |
Ovhi | + | - | - | - | - | - | - | - | - | - | - | - |
Ovhl | + | - | - | - | - | - | - | - | - | - | - | - |
Ovhs | + | + | - | - | - | - | - | - | - | - | - | - |
Omhi | + | + | + | + | + | - | - | - | - | - | - | - |
Omhl | + | + | + | + | + | - | - | - | - | - | - | - |
Ovli | + | + | + | + | + | + | + | + | - | - | - | - |
Ovll | + | + | + | + | + | + | + | - | - | - | - | - |
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Hao, Y.; Li, J.; Zhang, W.; Sun, M.; Li, H.; Xia, F.; Cui, H.; Bai, H.; Shi, L. Analysis of the Chemical Profiles and Anti-S. aureus Activities of Essential Oils Extracted from Different Parts of Three Oregano Cultivars. Foods 2021, 10, 2328. https://doi.org/10.3390/foods10102328
Hao Y, Li J, Zhang W, Sun M, Li H, Xia F, Cui H, Bai H, Shi L. Analysis of the Chemical Profiles and Anti-S. aureus Activities of Essential Oils Extracted from Different Parts of Three Oregano Cultivars. Foods. 2021; 10(10):2328. https://doi.org/10.3390/foods10102328
Chicago/Turabian StyleHao, Yuanpeng, Jingyi Li, Wenying Zhang, Meiyu Sun, Hui Li, Fei Xia, Hongxia Cui, Hongtong Bai, and Lei Shi. 2021. "Analysis of the Chemical Profiles and Anti-S. aureus Activities of Essential Oils Extracted from Different Parts of Three Oregano Cultivars" Foods 10, no. 10: 2328. https://doi.org/10.3390/foods10102328