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Spectrophotometric Determination of the Aggregation Activity of Platelets in Platelet-Rich Plasma for Better Quality Control

1
Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan
2
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
3
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
Dent. J. 2019, 7(2), 61; https://doi.org/10.3390/dj7020061
Received: 28 April 2019 / Revised: 29 May 2019 / Accepted: 30 May 2019 / Published: 3 June 2019
(This article belongs to the Special Issue Soft and Hard Tissue Regeneration)
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Abstract

Although platelet-rich plasma (PRP) is now widely used in regenerative medicine and dentistry, contradictory clinical outcomes have often been obtained. To minimize such differences and to obtain high quality evidence from clinical studies, the PRP preparation protocol needs to be standardized. In addition, emphasis must be placed on quality control. Following our previous spectrophotometric method of platelet counting, in this study, another simple and convenient spectrophotometric method to determine platelet aggregation activity has been developed. Citrated blood samples were collected from healthy donors and used. After centrifugation twice, platelets were suspended in phosphate buffered saline (PBS) and adenosine diphosphate (ADP)-induced aggregation was determined using a spectrophotometer at 615 nm. For validation, platelets pretreated with aspirin, an antiplatelet agent, or hydrogen peroxide (H2O2), an oxidative stress-inducing agent, were also analyzed. Optimal platelet concentration, assay buffer solution, and representative time point for determination of aggregation were found to be 50–100 × 104/μL, PBS, and 3 min after stimulation, respectively. Suppressed or injured platelets showed a significantly lower aggregation response to ADP. Therefore, it suggests that this spectrophotometric method may be useful in quick chair-side evaluation of individual PRP quality. View Full-Text
Keywords: platelet-rich plasma; platelets; aggregation; spectrophotometer; quality assurance platelet-rich plasma; platelets; aggregation; spectrophotometer; quality assurance
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Tsujino, T.; Isobe, K.; Kawabata, H.; Aizawa, H.; Yamaguchi, S.; Kitamura, Y.; Masuki, H.; Watanabe, T.; Okudera, H.; Nakata, K.; Kawase, T. Spectrophotometric Determination of the Aggregation Activity of Platelets in Platelet-Rich Plasma for Better Quality Control. Dent. J. 2019, 7, 61.

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