Copper Isotope Compositions of Superoxide Dismutase and Metallothionein from Post-Mortem Human Frontal Cortex
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Sex | Total CuSOD (ng) | δ65CuN (‰) 4 | Total CuMT (ng) | δ65CuS (‰) 4 | Δ65CuN–S (‰) 4 | Source |
---|---|---|---|---|---|---|---|
AD1 1,2 | Male | 180 | 0.34 | 43 | −0.25 | 0.59 | this study 5 |
AD2 1 | Male | 86 | 0.36 | 30 | −0.08 | 0.44 | 5 |
AD3 1 | Female | 40 | −0.33 | 27 | −0.39 | 0.06 | 5 |
CON1 | Male | 83 | 0.37 | 26 | −0.42 | 0.79 | 5 |
CON2 3 | Female | 48 | 0.31 | 47 | 0.14 | 0.17 | 5 |
YeastSOD–MT | 0.53 | [4] | |||||
RBCs–serum | Female | 0.46 | −0.24 | 0.60 | [12] | ||
RBCs–serum | Male | 0.67 | −0.28 | 0.95 | [12] |
Sample | Total Cu (ng) | δ65Cu (‰) | Δ65Cu (‰) |
---|---|---|---|
Bulk Brain | 635 | −0.09 | |
Metallothionein | |||
Replicate 1 | 43 | −0.17 | |
Replicate 2 | 37 | −0.21 | |
Replicate 3 | 45 | −0.37 | |
Mean (SD) | −0.25 (0.11) | ||
Superoxide dismutase | |||
Replicate 1 | 168 | 0.50 | |
Replicate 2 | 130 | 0.17 | |
Replicate 3 | 166 | 0.34 | |
Mean (SD) | 0.34 ** (0.17) | ||
SOD–MT Pair 1 | 0.67 | ||
SOD–MT Pair 2 | 0.38 | ||
SOD–MT Pair 3 | 0.73 | ||
Mean (SD) | 0.59 (0.17) |
Sample Code | Disease State of Brain | Sex | Age | PMI 1 |
---|---|---|---|---|
AD1 2 | Alzheimer’s disease | Male | 70 | |
AD2 2 | Alzheimer’s disease | Male | 75.4 | 18.5 |
AD3 2 | Alzheimer’s disease | Female | 76.8 | 31.0 |
CON1 | Healthy | Male | 76.9 | 49.0 |
CON2 3 | Healthy | Female | 76.8 3 | 40.3 3 |
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Larner, F.; McLean, C.A.; Halliday, A.N.; Roberts, B.R. Copper Isotope Compositions of Superoxide Dismutase and Metallothionein from Post-Mortem Human Frontal Cortex. Inorganics 2019, 7, 86. https://doi.org/10.3390/inorganics7070086
Larner F, McLean CA, Halliday AN, Roberts BR. Copper Isotope Compositions of Superoxide Dismutase and Metallothionein from Post-Mortem Human Frontal Cortex. Inorganics. 2019; 7(7):86. https://doi.org/10.3390/inorganics7070086
Chicago/Turabian StyleLarner, Fiona, Catriona A. McLean, Alex N. Halliday, and Blaine R. Roberts. 2019. "Copper Isotope Compositions of Superoxide Dismutase and Metallothionein from Post-Mortem Human Frontal Cortex" Inorganics 7, no. 7: 86. https://doi.org/10.3390/inorganics7070086