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Article

Chemical Profiling and Characterization of Different Cultivars of Cannabis sativa L. Inflorescences by SPME-GC-MS and UPLC-MS

1
Toscana Life Sciences Foundation, Via Fiorentina 1, 53100 Siena, Italy
2
Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, 20133 Milan, Italy
3
Phytochem Lab, Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, 20133 Milan, Italy
4
National Interuniversity Consortium of Materials Science and Technology (INSTM), Università degli Studi di Milano, 20133 Milan, Italy
5
Department of Drug Chemistry and Technology, Sapienza University, 00185 Rome, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Miguel Ángel Rodríguez-Delgado
Separations 2022, 9(4), 90; https://doi.org/10.3390/separations9040090
Received: 23 March 2022 / Revised: 31 March 2022 / Accepted: 31 March 2022 / Published: 2 April 2022
The chemical profile of the Cannabis sativa L. female inflorescences is rather complex being characterized by a large number of molecules belonging to different chemical classes. Considering the numerous applications in various fields, including the medical and pharmaceutical sectors, that have seen a large use of Cannabis genus in recent years, a precise characterization of the matrices is essential. In this regard, the application of adequate and suitable sampling and analysis techniques becomes important in order to provide an identification of the metabolites characterizing the profile of the sample under examination. The goal of this work is to provide additional information on the chemical composition of the inflorescences of five C. sativa different cultivars grown in Emilia Romagna (Italy) through the application of sophisticated analysis techniques such as Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry and Ultra-Performance Liquid Chromatography-Mass Spectrometry (SPME-GC-MS and UPLC-MS). The obtained data highlighted the presence of a high number of volatile and non-volatile compounds, thus allowing a comparative evaluation of the different samples. Furthermore, an in-depth statistical survey by Principal Components Analysis (PCA) and HeatMap, Hierarchical luster Analysis (HCA) and Partial Least Squares Discriminant Analysis (PLS-DA-VIP), was conducted to consider any correlations between the investigated cultivars. The findings of this study may help to provide more information on the C. sativa inflorescences useful for potential applications of their metabolites in scientific research. View Full-Text
Keywords: cannabinoids; non-cannabinoids; volatile and non-volatile compounds; chromatographic analyses; multivariate statistical analysis cannabinoids; non-cannabinoids; volatile and non-volatile compounds; chromatographic analyses; multivariate statistical analysis
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MDPI and ACS Style

Cicaloni, V.; Salvini, L.; Vitalini, S.; Garzoli, S. Chemical Profiling and Characterization of Different Cultivars of Cannabis sativa L. Inflorescences by SPME-GC-MS and UPLC-MS. Separations 2022, 9, 90. https://doi.org/10.3390/separations9040090

AMA Style

Cicaloni V, Salvini L, Vitalini S, Garzoli S. Chemical Profiling and Characterization of Different Cultivars of Cannabis sativa L. Inflorescences by SPME-GC-MS and UPLC-MS. Separations. 2022; 9(4):90. https://doi.org/10.3390/separations9040090

Chicago/Turabian Style

Cicaloni, Vittoria, Laura Salvini, Sara Vitalini, and Stefania Garzoli. 2022. "Chemical Profiling and Characterization of Different Cultivars of Cannabis sativa L. Inflorescences by SPME-GC-MS and UPLC-MS" Separations 9, no. 4: 90. https://doi.org/10.3390/separations9040090

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